摘要
为了解决以往赛加羚羊(Saiga tatarica)鉴别方法存在的不确定性或分辨率低的问题,在mtDNA的12S rRNA基因易变区设计了2对赛加羚羊特异性引物Saiga A和Saiga B,通过PCR扩增和常规电泳检测,分别得到127 bp和320 bp的片段,进而可通过这两个片段的有无来特异性检出赛加羚羊的DNA。引物Saiga A和Saiga B的最小检出量为1．0 ng DNA,稳定性和特异性好、灵敏度很高,可以应用于赛加羚羊样品的检测。
Two saiga-speeifie primer pairs, Saiga A and Saiga B, were designed at the variable region of 12S rRNA gene of mitochondrial genome aiming to solve the uncertainty or low resolution for already existed methods. A 127 bp and a 320 bp fragment were obtained respectively from these two primer pairs through PCR amplification. Then saiga DNA could be identified by the appearance of the two fragments through routine electrophoresis. The lowest positive quantity of template DNA was 1.0 ng for the two primer pairs. Results above indicate that this method with high stability, specificity and sensitivity can be applied to sample identification for saiga.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2006年第3期106-108,共3页
Journal of Northeast Forestry University
基金
黑龙江省科技攻关项目(GC01B107-01)
黑龙江省青年科学基金项目(QC02C28)资助。
