摘要
以风箱果幼苗顶芽为外植体,诱导不定芽,通过试管外生根技术建立植株再生体系。结果表明：以MS+BA1．0 mg·L^(-1)+NAA1．0 mg·L^(-1)为诱导培养基,MS+BA0．8 mg·L^(-1)+NAA0．4 mg·L^(-1)为增殖培养基,MS+IBA0．5 mg·L^(-1)+GA_30．3 mg·L^(-1)为壮苗伸长培养基,效果较好；试管外生根以100 mg·L^(-1)NAA处理30 min效果最佳,生根成活率为93．1%。
In vitro adventitious shoot was induced from apical bud of Physocarpus amurensis seedlings, and establishment of plantlet regeneration system was studied by ex vitro rooting of the in vitro regenerated microshoots. Results showed that the optimum culture media were obtained as MS ＋ BA 1.0 mg· L^-1 ＋ NAA 1.0 mg ·L^-1 for apical bud germination, MS ＋ BA 0.8 mg· L^-1 ＋ NAA 0.4 mg· L^-1 for shoot proliferation, and MS ＋ IBA 0.5 mg· L^-1 ＋ GA3 0.3 mg· L^-1 for shoot elongation and strengthening. The best treatment for ex vitro rooting was 100 mg· L^1 NAA soaked for 30 min, and the rooting and survival rate reached 93.1%.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2006年第3期42-44,共3页
Journal of Northeast Forestry University
基金
黑龙江省科技攻关项目(GB02B103)。
关键词
风箱果
组织培养
不定芽
试管外生根
植株再生
Physocarpus amurensis
Tissue culture
Adventitious shoots
Ex vitro rooting
Plantlet regeneration
