摘要
目的研究一种适用于水中过氧化氢检测的高灵敏度且特异性的测定方法。方法强氧化物在盐酸中可使碘化钾氧化产生定量的碘,此碘与淀粉溶液反应生成碘蓝,碘蓝的颜色在一定的浓度范围符合比耳定律。首先测定水样中过氧化氢和其他强氧化物的总量。然后利用过氧化氢在过氧化氢酶的作用下分解成O2和水,使过氧化氢失去氧化性的特点,再测定加过氧化氢酶除过氧化氢后样品中其他氧化物含量。根据2次测定结果之差计算样品中过氧化氢的含量。结果过氧化氢浓度在0～2μg/ml范围内有良好的线性关系,方法的RSD<1.2%,加标回收率98.7%～101.3%,最低检测浓度为0.08mg/L(0.08μg/ml)。结论该方法简便快速,灵敏度高,不受其他强氧化剂干扰,适用于水源水、饮用水等的痕量过氧化氢测定。
Objective To make a sensitive and specific method for quantitatively determination of hydrogen peroxide in water. Methods The strong oxide can oxidize potassium iodide to produce iodine in hydrochloric acid that reacts on amylum to produce iodine blue. In a range of concentration the colour of iodine blue accords with Bill＇ s law. Firstly the gross of hydrogen peroxide and the other strong oxides in the sample were determined. Secondly, on the action of catalase the hydrogen peroxide was decompounded into oxygen and water, then, hydrogen peroxide lost its characteristic of oxidation, the other oxides without hydrogen peroxide in the sample were determined. The difference of the two determined results is the content of hydrogen peroxide. Results The calibration curve was linear between 0 to 2 μg/ml （10 μg/5 ml）. The relative standard deviation was less than 1.2%. The rates of recovery were 98.7%-101.3%. The detection concentration was 0.08 mg/L（0.08 μg/ml）. Conclusion This method is simple, rapid, sensitive and hardly to be disturbed by any other strong oxidizers. This method is suitable for trace hydrogen peroxide analysis of source water and drinking water.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第3期258-261,共4页
Journal of Environment and Health
