摘要
目的:从细胞凋亡角度探讨当归多糖对豚鼠耳部银屑病样模型的作用。方法:50 g/L普萘洛尔乳剂外涂豚鼠耳背皮肤,制备银屑病样模型,然后随机分成模型对照组(Ⅰ)、低剂量组(Ⅱ)、中剂量组(Ⅲ)、高剂量组(Ⅳ),依次给予生理盐水、100,200,400 mg/kg当归多糖腹腔注射。应用末端标记技术(TUNEL)分别检测各组皮损治疗前后表皮角质形成细胞凋亡情况。结果:造模后豚鼠耳部上皮角质形成细胞凋亡指数与涂药前相比明显增加(P<0.05),Ⅱ、Ⅲ、Ⅳ组治疗后皮损凋亡指数显著高于治疗前(PⅡ<0.05,PⅢ<0.05,PⅣ<0.01)。结论:当归多糖对普萘洛尔诱发的豚鼠耳背银屑病样模型有治疗作用,其作用机理可能与其促进角质形成细胞的凋亡有关。
Objective： To investigate the influence of angelica polysaccharide on apoptosis in psoriasis- like lesions of guinea pigs. Methods： Propranolol emulsion （50 g/L） was applied on ears＇ back of Guinea pigs to produce the psoriasis-like models, then the forty guinea pigs randomly divided into four groups., model control group（ Ⅰ ）, low-dose group（ Ⅱ ）, mid-dose group（ Ⅲ ）and high-dose group（Ⅳ）. Normal saline and 100, 200 and 400 mg/kg angelica polysaccharide were injected intra-abdominally respectively. And in each group the apoptosis of keratinocytes in their skin lesions before and after treatment was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling（TUNEL） technique. Results： There was a large number of apoptosis keratinocytes in psoriasis-like lesions of guinea pigs＇ ears. Compared with that of normal guinea pigs, the apoptosis index was increased（P〈0.05）. Expression of apoptotic cells in psoriasis-like lesions after injecting angelica polysaccharide in group Ⅱ , Ⅲ and Ⅳ was significantly higher than that before treatments（PⅡ〈0.05 and PⅢ〈 0.05, PⅣ〈 0.01 ） respectively. Conclusion. Angelica polysaccharide might have a therapeutic effect on the psoriasis-like lesions in the epidermis of guinea pigs＇ ears induced by propranolol ointment, and the mechanism may be related to the increase in the frequency of apoptotic keratinocytes in lesions.
出处
《武汉大学学报（医学版）》
CAS
2006年第3期377-380,共4页
Medical Journal of Wuhan University
