摘要
目的:观察糖尿病造影剂肾损害(CIN)发病过程中氧化应激的作用以及肾皮质诱导性一氧化氮合酶(iNOS)活性的变化,并探讨厄贝沙坦对糖尿病CIN氧化应激和肾皮质iNOS活性的影响。方法:雄性Wistar大鼠56只,10只作为正常对照组(N组),其他46只作为糖尿病造模组。用腹腔单剂量注射链脲佐菌素建立糖尿病模型,饲养8周后糖尿病大鼠再随机分为3组:糖尿病对照组(DM组);糖尿病+造影剂组(CM组);糖尿病+厄贝沙坦+造影剂组(ARB组)。注入造影剂48 h后收集血、尿标本,测血肌酐(Scr)、内生肌酐清除率(Ccr)、尿微白蛋白排泄率(UAE)、尿N-乙酰--βD-氨基葡萄糖酐酶(NAG)。造影后第2天摘取肾脏左肾行组织病理学分析,右肾检测肾皮质内丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、iNOS活性。结果:与DM组比较,CM组注入造影剂后Scr、NAG升高,Ccr下降,肾皮质MDA含量升高,SOD、CAT活性下降,iNOS活性升高;与CM组比较,ARB组注入造影剂后Scr、NAG下降,Ccr升高,肾皮质MDA含量下降,SOD、CAT活性升高,iNOS活性下降。结论:氧化应激参与了糖尿病CIN的发病过程;厄贝沙坦对糖尿病CIN有保护作用,此保护作用可能与之抑制CIN的氧化应激有关。
Objective： To investigate the effect of irbesartan on oxidative stress and inducible nitric oxide synthase （iNOS） in diabetic rats with contrast induced nephropathy（CIN）. Methods： Streptotocin-induced diabetic rats were fed for eight weeks and normal control group （N group）, diabetic group （DM diatrizoate （CM group） and diabetic rats treated with then randomly assigned into four groups as group）, group of diabetic rats injected with irbesartan before diatrizoate injection （ARB group）. Serum creatinine（Scr） ,glomerular filtration rate（GFR）, urinary albumin excretory rate （UAE）, N-acetyglocosamidase（NAG）, lipid peroxidation （MDA）, antioxidant enzymes including superoxide dismutase（SOD）, catalase （CAT） and iNOS activity in renal cortex were measured 48 hours after diatrizoate injection. Results： Compared with DM group, the Scr, NAG activity, MDA concentration and iNOS activity in renal cortex were higher and Ccr,SOD and CAT activity in renal cortex were less in CM group. Compared with CM group, the Scr, NAG activity,MDA concentration and iNOS activity in renal cortex were less and Ccr, SOD and CAT activity in renal cortex were higher in ARB group. Conclusion： Irbesartan has renal protection on contrast induced nephropathy in diabetic rats, probably due to suppression of renal local oxidative stress.
出处
《武汉大学学报（医学版）》
CAS
2006年第3期354-357,i0001,共5页
Medical Journal of Wuhan University
关键词
糖尿病
造影剂肾损害
厄贝沙坦
Diabetes Mellitus
Contrast Induced Nephropathy
Irbesartan
