摘要
目的:探讨过敏性哮喘患者和健康人外周血单核细胞来源树突状细胞(DC)负荷抗原后表达组成性趋化因子受体(CCR7)和MHCⅡ类分子(HLA-DR)的差异,为进一步研究过敏性哮喘的发病机制及治疗提供新的思路。方法:分别从过敏性哮喘患者(哮喘组)和健康人(对照组)外周血分离单个核细胞并获取贴壁细胞,经重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和重组人白细胞介素-4(rhIL-4)体外培养为未成熟DC,加入Der p 1使其负荷抗原;观察培养的DC的形态变化,并用流式细胞仪检测两组负荷抗原的DC表面趋化因子受体(CCR7)及MHCⅡ类分子(HLA-DR)的表达。结果:与对照组相比,哮喘组负荷抗原的DC表达较高水平CCR7(P<0.01)及HLA-DR(P<0.01);两组CCR7和HLA-DR均呈正相关(哮喘组r=0.73,P<0.05;对照组r=0.76,P<0.05)。结论:由于过敏性哮喘患者负荷抗原的DC与健康人相比在趋化因子受体及成熟标志物表达方面存在明显差异,从而趋化因子反应性及成熟情况存在差异,后者成为哮喘发生的重要机制之一。
Objective： To explore the difference in expression of constitutive chemokine receptors and MHC Ⅱ molecules expressed by Der p 1-pulsed monocyte-derived dendritic cells （DCs） generated from both healthy donors and asthmatic patients sensitive to Dermatophagoides pteronyssinus （Dpt）, and to establish experimental foundation for further research of pathogenesis and therapeutics in allergic asthma. Methods： Mononuclear cells were isolated from peripheral blood of healthy donors and asthmatic patients, and the adherent cells were harvested and cultured with the combination of rhGM-CSF and rhlL-4 as immature DCs （iDCs）, then the generated cells were incubated for 24 hours with 1 mg/L Der p 1 （allergen of Dermatophagoides pteronyssinus）. The expression of CCR7 and HI.A-DR on Der p 1-pulsed DCs was analyzed by flow cytometry （FCM）. Results： Compared with control group, Der p 1-pulsed DCs from asthmatic group had increased the expression of CCR7 （P〈0.01） and HLA-DR （P〈0.01）. There was positive correlation between CCR7 and HLA-DR in both groups （both P〈0.05）. Conclusion： Because of increased expression of CCR7 and HLA-DR on Der p 1-pulsed DCs from asthmatic patients, there is a difference in chemokine responsiveness and maturation of DCs between asthmatic patients and healthy donors, which is one of important pathogenesis for allergic asthma.
出处
《武汉大学学报（医学版）》
CAS
2006年第3期349-353,共5页
Medical Journal of Wuhan University
