摘要
目的探讨生长抑素（SST）及其类似物奥曲肽对急性呼吸窘迫综合征（ARDS）大鼠的治疗作用．方法百草枯灌胃法制作大鼠ARDS模型，灌注百草枯后24h，经尾静脉输入SST（1mg／kg）、奥曲肽（0．1mg／kg）或注射地塞米松（1mg／kg），观察动物肺组织病理学、血气、肺系数等改变，同时测定大鼠血浆、肺泡灌洗液（BALF）及小肠组织匀浆液肿瘤坏死因子α（TNF—α）及白细胞介数6（IL-6）的含量．结果输注SST、奥曲肽或注射地塞米松后，与ARDS模型组相比，大鼠肺组织炎症病变明显减轻，动脉血氧分压（PaO2）有升高趋势（升高75％～84％），二氧化碳分压有降低趋势（降低17％～22％），血浆pH值回升，肺系数有降低趋势（降低13％～15％）．血浆TNF—α水平与ARDS模型组比较，有降低趋势，但差异无统计学意义（P〉0．05）。肺泡灌洗液及小肠匀浆液TNF—α水平SST组、奥曲肽组及地塞米松组较ARDS模型组降低（P〈0．05）．SST组、奥曲肽组及地塞米松组肺泡灌洗液IL-6水平较ARDS模型组均有降低趋势，但差异无统计学意义（P〉0．05），血浆及小肠匀浆液IL-6水平各组间差异无统计学意义。结论SST及其类似物奥曲肽有改善ARDS症状，减低肺炎症病变及TNF—α含量的作用，可作为ARDS治疗的辅助药物。
Objective This study was aimed to assess the effects of somatostatin （SST） and its analog Octreotide on the treatment of acute respiratory distress syndrome （ARDS） in rat model. Methods ARDS rat model was induced by administration of paraquat. After 24 hours oral administration of paraquat, three drugs （SST, Octreotide and dexamethasone at dosage of 1 mg/kg, 0. 1 mg/kg and 1 mg/kg respectively） were infused into the tail veins of rats so as to form three treatment groups. The rats given only paraquat were subjects in the ARDS group. PaO2 level and pulmonary coefficient were detected, and the morphological changes of lungs were examined. In addition, TNF-α and IL-6 levels in plasma, Bronchoalveolar lavage fluids （BALF） and intestine homogenate were measured respectively. Results The ARDS rats treated with SST, Octreotide and dexamethasone showed mitigation of inflammatory response in the lungs, improvement in lung ventilation, reduction of pulmonary coefficient （-13%-15%）, increase in PaO2 level （＋75%-84%）, decrease in PaCO2 level （-17%-22%）, and restoration of pH value to normal in plasma, when compared with the rats of ARDS group. TNF-α level in plasma tended to decline （P〉0. 05） ; it significantly lowered in BALF and intestine homogenate in the ARDS rats treated （P〈 0. 05）. IL-6 level in BALF tended to be lower in treatment group （P〉0. 05）, but the IL-6 level in plasma and intestine homogenate displayed no significant difference between the treatment group and ARDS group. Conclusions These results suggest that SST and its analog Octreotide assume a role in improving the clinical manifestation of ARDS, alleviating the inflammatory condition of the lungs, and reducing the TNF-α level in the BALF and intestine homogenate, therefore they may be useful adjuvants in the treatment of ARDS.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第3期412-415,共4页
Journal of Sichuan University(Medical Sciences)
基金
四川省科技厅攻关项目(03SG022-006-2)
四川大学校基金资助
关键词
急性呼吸窘迫综合征
生长抑素
奥曲肽
Acute respiratory distress syndrome Somatostatin Octreotide
