摘要
目的研究镁对离体培养的缺氧大鼠脑皮质神经元的影响。方法将大鼠皮质神经元分别给予MgSO4和Mg2+-ATP处理后连续给予97.5%N2和2.5%CO2混合气体,制成大鼠皮质神经元体外缺氧试验模型,分别观察缺氧后1、2、4h不同时间神经元死亡率、神经细胞内Ca2+浓度(Fluo-3标记,激光共聚焦显微镜检测)的动态变化和培养液中LDH、K+、NSE浓度的变化。结果MgSO4和Mg2+-ATP能减少缺氧状态下的脑皮质神经元钙内流(P<0.05),降低培养液中LDH、K+、NSE浓度(P<0.05),明显降低神经元死亡率(P<0.05)。结论镁对离体培养的皮质神经元缺氧损伤有肯定的保护作用。
Objective This is an in vitro study aimed to assess the influence of magnesium on cultured anoxic cortical neurons of rats. Methods After being treated with MgSO4 and Mg^2＋-ATP respectively, the cortical neurons of rats were given mixture of 97. 5% N2 and 2.5% CO2 continuously to set up the model of anoxic cortical neuron of rats at different time points. The mortality of neurons, the levels of Ca^2＋ within nerve cells （marked by Fluo-3, detected by laser confocal microscopy）, and the levels of lactate dehydrogenase （LDH）, K^＋ and neuron specific enolase （NSE） in the culture fluid were investigated at 1,2, 4 hours after the inception of anoxia. Results MgSO4 and Mg^2＋-ATP reduced the calcium influx of anoxic cortical neuron （ P〈0. 05）, lowered the level of LDH, K^＋ , NSE in the culture fluid （P〈0.05） and significantly decreased the mortality of neurons（ P 〈0. 05）. Conclusion The protective efficacy of magnesium against the anoxic damage to cultured cortical neurons of rats is corroborated.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第3期387-390,411,共5页
Journal of Sichuan University(Medical Sciences)
关键词
镁
神经元
缺氧
激光共聚焦显微镜
Magnesium Neuron Nypoxia Laser confocal microscopy
