摘要
Ethylene plays an extensive role in plant growth and development.. 1-aminocyclopropane-1-carboxylate (ACC) oxidase (ACO) is the key enzyme in ethylene biosynthesis. In this study, a 354 g DNA and a 213 bp cDNA base pair (bp) candidate fragment was amplified from pepper with primers derived from the ACO sequence (AJ011109) reported by Ernesto. The putative new gene was analyzed by bioinformatics tools.
乙烯作为一种植物激素,在植物生长发育过程中发挥着重要的作用。1-氨基环丙烷-1-羧酸(ACC)氧化酶(ACO)是乙烯生物合成途径中的一种关键酶。本研究以辣椒为研究材料,根据报道的辣椒ACC氧化酶基因序列(AJ011109)设计引物,利用PCR的方法从中国种湘研4号基因组获得长度为354bp的gDNA序列和213bp的cDNA序列。利用生物信息学软件对所获得的gDNA和cDNA序列进行了分析。