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Isolation and analysis of pepper ACC oxidase gene partial sequence

辣椒ACC氧化酶基因部分序列的分离与鉴定(英文)
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摘要 Ethylene plays an extensive role in plant growth and development.. 1-aminocyclopropane-1-carboxylate (ACC) oxidase (ACO) is the key enzyme in ethylene biosynthesis. In this study, a 354 g DNA and a 213 bp cDNA base pair (bp) candidate fragment was amplified from pepper with primers derived from the ACO sequence (AJ011109) reported by Ernesto. The putative new gene was analyzed by bioinformatics tools. 乙烯作为一种植物激素,在植物生长发育过程中发挥着重要的作用。1-氨基环丙烷-1-羧酸(ACC)氧化酶(ACO)是乙烯生物合成途径中的一种关键酶。本研究以辣椒为研究材料,根据报道的辣椒ACC氧化酶基因序列(AJ011109)设计引物,利用PCR的方法从中国种湘研4号基因组获得长度为354bp的gDNA序列和213bp的cDNA序列。利用生物信息学软件对所获得的gDNA和cDNA序列进行了分析。
出处 《Agricultural Science & Technology》 CAS 2006年第1期2-7,共6页 农业科学与技术(英文版)
关键词 ACC oxidase CLONING PCR BIOINFORMATICS ACC氧化酶 克隆 PCR 生物信息学 植物激素 辣椒
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参考文献14

  • 1[1]Ernesto G P,Edmundo L G.1999.Induced gene expression of 1-aminocyclopropane-1-carboxylic acid in pepper (Capsicum annuum L.) by arachidonic acid.Plant Science,145,11-21.
  • 2[2]Gross K C,Watada A E,Kang M S,Kim K S,Lee S W.1986.Biochemical changes associated with the ripening of hot pepper fruit.Phsiol.Plant,66,31-36.
  • 3[3]Hamilton,A.J.,Lycett,G.W.and Grierson,D.1990.Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants.Nature,346,284-287.
  • 4[4]Kim W T,Yang S F.1994.Structure and expression of cDNAs encoding 1-aminocyclopro-pane-I-carboxylate oxidase homologs isolated from excised mung bean hypocotyls.Planta,194,223-229.
  • 5[5]Lasserre E,Bouquin T,Hernandez J A,et al.1996.Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melon L.).Mol.Gen.Genet.,251,81-90.
  • 6[6]Lu G,Yang S F,Liang H,Lu Z.1990,Changjiao hot pepper are nonclimacteric.HortScience,25,807.
  • 7[7]Lurie S,Ben Yehoshua.1986.Changes in membrane properties and abscisic acid during senescence of harvested bell pepper fruit.J.Am.Soc.Hortic.Sci.,111,880-885.
  • 8[8]Pretel M T,Serrano M,Amoros A,et al.1995.Non-involvement of ACC and ACC oxidase activity in pepper fruit ripening.Postharvest Biology and Technology,295-302.
  • 9[9]Rhodes M J.1980.The maturation and ripening of fruits.In:K.Thimann (Editor),Senescence in Plants,157-205.
  • 10[10]Saltveit M E Jr.1977.Carbon dioxide,ethylene and color develpoment in ripening mature green bell peppers.J.Am.Soc.Hortic.Sci.,102,523-525.

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