摘要
根据已发表的外膜蛋白基因核苷酸序列设计一对特异性引物,应用PCR方法扩增出禽巴氏杆菌5:AC48-1株的ompH全长片段,将ompH进行T-A克隆、序列测定和分析。结果表明,ompH全长1597bp,含有一个1056bp的开放性阅读框架(ORF),编码352个氨基酸,前20个氨基酸组成信号肽。与已知的15个血清型及疫苗株CU的ompH的核苷酸及其推导的氨基酸序列比较,核苷酸同源性在51.5%～98.7%之间,氨基酸同源性在39.3%～98.7%之间。氨基酸多序列比较显示,血清型特异性抗原表位位于60～80位和200～220位氨基酸处。
According to ompH of pasteurella multocida published in GenBank, a pair of primers were designed. Using the primers, full-length ompH of C48-1 was amplified by PCR. It was cloned into pMD18-T vector and then sequenced. The result showed that the full-length ompH composed 1 597 bp.The coding region of ompH is 1056 bp at length. The predicted primary protein is composed of 352 amino acid with a 20-amino-acid signal peptide, After comparing with ompH from 15 pasteurella multocida serotype as well as the CU vaccine strain, it showed that nucleotide sequence homologies were 51.5 % to 98,7 % and the deduced amino acid homologies were 39,3% to 98.7%. By amino acid sequence alignment of ompH, two hypervariable regions-amino acids 60 to 80 and 200 to 220 which contain serotype specific epitomes and may induce protective antibodies were identified.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第3期253-256,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
广东省自然科学基金资助项目(3227)
关键词
禽巴氏杆菌
外膜蛋白
序列分析
同源性
avian pasteurlla multocida
outer membrane protein
sequence analysis
homology
