摘要
用流行性腮腺炎(流腮)病毒Enders株接种鸡胚尿囊腔培养,尿囊液经聚乙二醇6000处理制备流腮病毒抗原,用ELISA法检测流腮患者血清中特异性IgM抗体,其敏感性,特异性、重复性和稳定性都很高。 79份流腮患者血清,检出特异性IgM72份,阳性率为91％,32例非流腮患者IgM全部阴性、两者有极显著差异(P<0.01)。 10份血清作血清倍比稀释至1∶3200测IgM仍全部阳性,1∶6400稀释仅1例阴性,1∶12800稀释5例中仍有2例阳性。 10份血清作流腮抗原特异性抗体阻断试验,光密度抑制率均大于50％,平均为87％,10份标本作2-ME和SPA阻断后检测IgM抗体,结果2-ME阻断标本全部阴转,而SPA阻断标本仍阳性,证实所检测为流腮特异性抗体。 24份标本2次重复检测流腮IgM,其阴、阳性结果一致,这期间抗原放4℃ 1个月,提示抗原的稳定性和方法的重复性都很好。本方法敏感性明显高于血凝抑制试验,其阳性率分别为91％和61％,两者有显著差异。而且所用试剂简单经济,操作简便,快速,适用于临床早期诊断,易于广泛推广应用。
Specific IgM from patients with epidemic parotitis ( EP ) was detected by enzyme linked immunosorbent assay (ELISA). The mumps virus antigen was prepared by using mumps virus Ender's strain. The detected IgM antibody was confirmed by 2-EM destruction. Sensitibity of the assay for mumps virus-IgM was high as showing a titer of 1:12800 in the positive sera from the patients. In clinically diagnosed 79 cases from the patients with epidemic parotitis, 72 cases were IgM positive, and the positive rate was 91%. In the 32 sera from the control patients with measles, VZV, rabies and others, all of them were negative by our method.
出处
《病毒学杂志》
CSCD
1990年第4期362-366,共5页
关键词
流行性
腮腺炎
IGM抗体
ELISA
Epidemic parotitis
Specific IgM antibody
Enzyme linked immunosorbent assay
