摘要
1988年初上海发生甲肝暴发流行,流行病学分析与生食毛蚶有关,本文报道在引起甲肝暴发流行的毛蚶产地江苏启东海域采集毛蚶标本,取腮和消化系统制成40％悬液,用FRhk_4细胞分离甲肝病毒,每代培养21天,连续传二代后经过免疫荧光,ELISA,HAVeDNA斑点杂交试验,免疫电镜等鉴定,5份标本全部阳性,上述结果证实毛蚶中携带甲肝病毒,并为病原学研究提供依据。
An outbreak of hepatitis A with uncooked Arca subcrenata Lischke has been reported in shanghai in early 1988. Five specimens were collected from the sea at Qidong County Jiangsu Province vhere Produce Arca subcrenata Lischke. Specimens were made of suspension of 40% with the gill and system enteron, then treated by trichloromethane. HAY was isolated with FRHK4 cell cultrues by 21 days and passed continually two generation. All specimens are positive for HAY by IF, ELISA, HAV-cDNA dot hybridization test and IEM. It has been shown Arca subcrenata Lischke carries HAV, which can provide evidence for etiology study.
出处
《病毒学杂志》
CSCD
1990年第3期240-244,共5页
关键词
甲型肝炎病毒
毛蚶
Hepatitis A Virus FRhk4 cell Atca subcTenata Lischke
