摘要
1986年先后在北京的公园采到菊花病样4个,北京动植检所送检风信子球茎病样一个。这5个分离物经人工接种10科36种植物,多表现花叶、斑驳、畸形等症状,而番茄的果实小而无籽。以菊-6分离物制备的抗血清与其它4个分离物产生明显愈合的沉淀线,证明它们的抗原性完全相同,这5个分离物与从菜豆分离的番茄不孕病毒(TAV)的抗血清反应产生分枝状的明显沉淀线。菊-6分离物的理化特性:TIP为55—60℃,DEP为10^(-2)—10^(-4),LIV为2天。其病毒粒子的平均直径为27nm等轴多面体,0.D260/280=1.76,病毒衣壳旦白分子量约为26300。根据上述以菊-6分离物为代表的5个分离物的特性与CMI/AAB Descriptions ofPlant Viruses No.79.1971所描述的从菊花分离的TAV基本相同,鉴定它们属TAV,但与菜豆分离物不完全相同,属不同株系。
Four diseased samples of chrysanthemum showing chlorotic mosaic or green flowers were collected from the Beihai Park and Zizhuyan Park in Beijing in July and December of 1986.Another one showing black necrotic spots on bulb of Hyacinth was from Beijing Animal and Plant Quarantine Service. These five isolates were inoculated on 36 species of plants belonging to 10 families(e.g. Solanaceae, Leguminosae, Chenopodiaceae, Cucurbitaceae, Amaranthaceae and Compositae etc.). The infected plants showed either systemic mosaic, severe mottle or malformation. The diseased fruits of tomato became small,deformed and seedless. Cucumber and cowpea showed chlorotic local lesions. They did not infect Phaseolus vulgaris , Psoralea corylifolia and Lavatera tremestris. The stability of the virus was: TIP 55-60℃, DEP 10^(-2)-10^(-4), LIV 2 days(room temperature). The purified virus was shown as isometric polyhedron particles with a diameter of about 27 nm. The virus has typical ultraviolet absorption spectrum of nucleic protein, O.D 260/280=1.76, its moleclar weight was 26300. By agar double diffusion assay, the precipitation band between the antiserum of bean isolate(TAV) and each of the antigens of the 5 isolates from Chrysanthemum was branching, while the precipitation band between the antiserum of isolate Chry-6 and each of the antigens of the 5 isolates(including Chry-6) was confluent. Based on these characteristics as well as on the results of biological identification, the five isolates coincided closely with the Tomato Aspermy Virus recorded on CMl/AAB Descriptions of Plant Viruses No. 79. 1971. So it is concluded that these five isolates were identical with Tomato Aspermy Virus, but were not of the same strain with the bean isolate.
出处
《病毒学杂志》
CSCD
1990年第2期186-192,共7页
基金
农业部"七五"重点科研项目的专题
关键词
番茄不孕病毒
菊花
风信子
鉴定
Tomato Aspermy Virus
Chrysanthemum hyacinth
Isolates
Identification
