摘要
用八株不同来源的流行性出血热(EHF)病毒的单克隆抗体(McAb),采用血凝抑制试验、反向间接血凝抑制试验、间接酶联免疫及阻断酶联免疫试验等,对两种方法(TE、SA)制备的血凝素抗原进行分析。根据A35、2A6McA5试验的结果,证实血凝素抗原中的核蛋白上存在有非构象依赖性的血凝结合位点;而另外5株McAb在血凝抑制活性方面,虽具有明显的株间交叉,但在反向间接血凝抑制试验、间接酶联免疫试验时则均为阴性,故认为其血凝结合位点位于病毒的膜蛋白,可能与G_2糖蛋白有关,为构象依赖性位点。有关4G6McAb,在阻断酶联免疫试验时,虽与A35、2A6一样具有较高的阻断率,但在反向间接血凝抑制试验、间接酶联免疫试验时明显有别于后二者,对其属性有待进一步确定。
Eight monoclonal antibodies(Mc Abs) from various epidemic hemorrhagic fever(EHF) virus have been used for analyzing the epitopes of SA and TE hemagglutinin antigens by hemagglutination inhibition(HI), reversed passive hemmagglutination inhibition(RPHI), indirect ELISA and blocking ELISA.It was demonstrated that there were conformation independing hemagglutination binding epitopes in nucleocapsid protein by A_(35)、2A_6 McAb. Although other five strains of McAbs possessed obvious crosses in the activity of hemagglu tinative inhibition, they were negative in RPHI, ELISA.So it is possible for their hemagglutination binding epitopes to localize in membrane protein of virus and to relate with glycoprotein G_2, conformation depending epitopes. Another McAb, 4G_6 was similar to A_(35)、2A_6 that had higher blocking rate,but evidently it differed from the latter in RPHI, ELISA. Its properties will be studied further.
出处
《病毒学杂志》
CSCD
1990年第2期125-129,共5页
基金
国家自然科学基金资助的课题
关键词
流行性出血热
病毒
血凝素抗原
Epidemic hemorrhagic fever virus
Monoclonal antibody
Hemagglutinin
Epitope analysis
