摘要
本文报道了BmCPV感染家蚕细胞系后的电镜观察。病毒感染早期,细胞质内形成电子致密的病毒发生基质,由病毒发生基质形成BmCPV球状病毒粒子;病毒感染48小时后,多角体在病毒发生基质周围形成,大量的病毒粒子随机包埋在多角体内;病毒接种后96小时,多角体数目增多,其形状有三角,四角,五角及六角形,细胞质内充盈多角体致使细胞核被挤向细胞一侧并伴有形态的改变,受染细胞约为40％。
The paper reports observations on a cytoplasmic polyhedrosis virus (CPV) from Bombyx mori infecting Bombyx mori cells (Bm-N)by electron microscopy. In the early stage of infection, the electron dense virogenic stroma containing virions formed in the cytoplasm of infected cells. Inclusion bodies appea- red at periphery of the virogenic stroma at 48hr after infection. The large number of virions randomly embedded within the polyhedrin matrix. Polyhedra were tri-gonal, tetragonal, pentagonal and hexagonal in shape. By 96hr after infection the cytoplasm of infected cells filled with polyhedra and the nucleus of infectcd cells deformed.
关键词
家蚕
CPV
细胞系
Bombyx mori Cytoplasmic polyhedrosis virus Bm cell line
