摘要
应用基因重组技术,将EB病毒早期抗原(EA)P138两个抗原部位的基因片段和早期抗原P54的完整基因重组,构建成质粒pUC B,转化大肠杆菌JM109获得高效表达。其产物为分子量83kD的P83。经Western blot检验证实,P83能与鼠抗P138和鼠抗P54两种单克隆抗体产生特异性反应。用P83检测30份鼻咽癌病人血清IgA类抗体100％阳性,30份正常人血清全部阴性。
A plasmid pUC B encoding both antigenic sites of Epstein-Barr virus early protein 138(Pl38)and whole P54 was successfully constructed. Both proteins were expressed very well as β-galactosidase fusion in Escherichia coli JM 109.The molecular weight of this fusion protein is shown to be 83kDa. By immunoblotting, this P83 could react with mouse monoclonal antibodies against EBV P138 and P54, but not with unrelated monoclonal antibody. IgA/P83 antibodies were detected in the sera of 30 patients with nasopharyngeal carcinoma but not in the sera of 30 normal individuals.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第4期316-321,共6页
Chinese Journal of Virology
关键词
EB病毒
早期蛋白质
鼻咽癌
Epstein-Barr virus Early protein Plasmid pUC B Nasopharyngeal carcinoma
