摘要
肾综合征出血热(HFRS)为一组抗原性密切相关的布尼亚科汉坦病毒引起的急性传染病。在我国存在至少两种临床表现、动物宿主及流行特征截然不同的血清型别,即血清Ⅰ型(汉坦型)和血清Ⅱ型(汉城型)。这两型病毒间的血清学定型已有报道。近年来,除啮齿类动物外,从临床病人以及非啮齿类动物体内也分离到了HFRS病毒。同时出现两类型别毒株共存,以及从家鼠体内分离到野鼠型毒株或从野鼠体内分离到家鼠型毒株的复杂情形。为此,准确检定并鉴别不同来源毒株型别,将为深入了解其病原学、流行病学以及制定疫苗生产策略提供重要信息。
Seven Hantavirus strains isolated from different animal hosts and geographic regions were differentiated by polymerasc chain reaction ( PCR ) assay using the synthetic strain-specific oligoaucleotide primers designed with a region from 30 to 748 nucleotides of the M segment of HTN and R22 strains. 76-118-specific primers amplified only the RNAs extracted from Z20, J3 strains isolated from Apodemus agrarius in Zhejiang Province, HTN strain in Korea,and S2 strain from Suncus murinus in the suburbs of Shanghai. In contrast, R21-specific primers amplified only the RNAs extracted from R22, K24 strains isolated from Raitus norvegicus in Henan Province and SR11 strain in Japan. The identification of the generated DNA products was verified by dot-hybridization with internal probes specific for the M segment of HTN and R22 strains. As a new sensitive and specific molecular technique, PCR assay offers an opportunity to identify and differentiate the serotypes of Hantavirus strains in China quickly and accurately.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第4期368-372,共5页
Chinese Journal of Virology
关键词
HFRS
聚酶链反应
鉴别
Hantaviruses Polymerase chain reaction Differentiation
