摘要
以小麦全蚀菌病毒泰安株(Gaeumannomyces graminis virus Taian strain,GgV-Taian)的dsRNA为探针进行斑点杂交,比较低了5种dsRNA真菌病毒和7株小麦全蚀菌病毒分离物间的同源性。GgV-Taian dsRNA与米曲霉病毒、黑曲霉病毒、美味侧耳病毒的dsRNA有杂交反应;而与草菇病毒dsRNA无反应;与5株GgV分离物均有反应。与无血清学关系的黑曲霉病毒也有杂交反应。本文提供了寄主分类地位甚远的真菌病毒有同源序列的新证据。
Using labelled dsRNA of Gaenmannomyces 9raminis virus Taian ( GgV-Taian ) as probe, dot blot analysis was made between GgV-Taian and viruses from five species of fungus or seven isolates of GgV. The dsRNA of GgV-Taian hybridized with viral dsRNA of distantly related species of As-per3illus oryzae, A. niger, Pleurotus sapid us and dsRNA of GgV collected from Taian, Yantai, Jiangsu, Zhejiang; but not with dsRNA of GgV-Hubei, GgV-Qinghai and Volvariella volvacea virus.Comparison of the results of the dot blot hybridization with those of the serological test among these viruses showed that the antiserum to GgV-Taian reacted with viruses of A, oryzae and P. sapidus, but not with A, niger virus. However, there was strong cross hybridization between A. ni-9er virus and GgV-Taian. The results give new evidences of the homology of viruses in taxonomi cally distantly related fungal species.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第3期245-249,共5页
Chinese Journal of Virology
基金
国家自然科学基金
关键词
真菌病毒
小麦全蚀菌
病毒
同源性
Mycoviruses Gaeumannomyces graminis dsRNA hybridization Homology
