摘要
运用基因重组技术在大肠杆菌生产出有诊断意义的人免疫缺损病毒(HIV)蛋白,将其初步提纯,再用快速蛋白液相层析技术进一步纯化,即可直接点样至硝酸纤维膜,用来检测HIV抗体。实验显示,这种免疫斑点法(Immunodot assay,IDA)的敏感性与间接免疫荧光法(IIF)相同,而特异性比它高,与蛋白印迹法(WB)相近。由于IDA试剂成本比IIF低,可用于HIV抗体检测的初筛。与IIF相比,IDA法具有假阳性率低、试剂稳定性高、操作简便和不需要昂贵的荧光显微镜等优点。因而是一种适合在基层应用的方法。
Genetic recombinant human immunodeficiency virus ( HIV ) proteins, gp41 and p24 were purified by several steps including Superose 12 chromato-graphy. An immunodot assay ( IDA ) for HIV antibody detection was dev-eloped with the purified proteins and tested with a panel of positive and negative sera. The results showed equal sensitivity and greater specificity compared with immunofluorescent assay. With the advantage of low costs in manufecturing, stable for postal distribution, simple in performance and no need of sophisticated equipment nor highly trained technical staff, IDA is a suitable test for serological screening for HIV antibody, especially in field studies.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第3期250-255,共6页
Chinese Journal of Virology
