摘要
通过1％琼脂糖电泳和十六烷基溴代三甲胺介导RNA相转移分离法,将水稳齿叶矮缩病毒基因组分为5个组份,基因组双链RNA经羟甲基汞变性后,在兔网状红细胞溶胞物翻译体系中,能高效地表达病毒蛋白质。每个RNA片段至少能编码一种蛋白蛋,但各个片段的翻译活性有明显差异。分析了该病毒基因组各组份与其表达产物的对应关系。
Rice ragged stunt virus is a plant reovirus with a genome of 10 ds-RNA segments. By means of 1% agarose gel elec trophoresis and hexadecyl-tri-methy-arnmonium- bromide mediated phase-transferring extraction, the genome of the virus could be divided into 5 fractions. In the in vitro translation system derived from rabbit reticulocyte lysate, the 5 fractionated ds-RNAs could act as translation templates after being denatured by 0.5% hydro-methylmercury. The incorporation of 35S-Met in translation products differed from fraction to fraction, and each RNA segment coded at least one virus-specific protein. The genome coding assignment of the virus was elucidated by analysing the in vitro translation products of the viral ds-RNA fractions.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第2期167-172,共6页
Chinese Journal of Virology
基金
国家自然科学基金
关键词
RRSV
呼肠孤病毒
基因组
分离
Plant reovirus Genomic RNA fractionation In vitrotranslation Genomic coding assignment
