摘要
应用抗肾综合征出血热病毒(HFRSV)R22、76—118及Hallnas株的10株单克隆抗体的免疫荧光反应发现,Z-20及SR-11病毒株的反应分别类同于野鼠型和家鼠型病毒株.用这两株病毒接种Vero-E6细胞获得的血凝素做血凝抑制(HI)试验证实,来自浙江的肾综合征出血热病人血清,对Z-20株病毒血凝素的HI抗体平均滴度比对SR-11高6.62倍,而来自河南及山西的病人血清,则对SR-11的HI抗体平均滴度分别高于对Z-20血凝素的4.93和4.67倍,来自上海的病人血清,对两株病毒血凝素的HI抗体滴度差异仅为2 11倍.根据血凝抑制试验,有可能对我国不同地区来源的肾综合征出血热病人血清进行分型。
Two strains of hemorrhagic fever with renal syndrome ( HFRS ) viruses, SR-11 and Z-20, were demonstrated to be Rattus-and Apod emus-associated serotypes respectivsly by immunofluorescent assay with 10 monoclonal antibodies against R-22, 76-118 and Hallnas strains.Hemagglutinins were prepared with the two strains propagated in Vero-E6 cell lines, and 246 serum samples of HFRS patients from different locations were tested for hemagglutination inhibition ( HI ) antibodies. The GMT of HI antibodies to SR-11 in the sera from Henan and Shanxi Provinces were by 4 times higer than that to Z-20,On the other hand,in the sera from Zhejiang Province, the GMT of HI antibodies to Z-20 were by 4 times higer than that to SR-11. However,only 2.11 times difference was observed in HI antibody titers to the two hemagglutinius in the sera from Shanghai, where both Rattus-and Apod emus-associated diseases were considered to be exist. Thus, the HI assay with the two strains(SR-l 1 and Z-20)may offer a possibility of identifying serotypes of HFRS patients in different locations of China.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第1期62-67,共6页
Chinese Journal of Virology
关键词
肾综合征
出血热
血清型
血凝抑制
Hemorrhagic fever with renal syndrome Serotypes Hera-agglutination inhibition assay
