摘要
目的检测食管鳞状细胞癌(ESCC)中p53基因突变和蛋白表达及其与临床病理和预后的相关性。方法采用聚合酶链反应-单链构象多态性分析(PCR-SSCP)和免疫组织化学(SP)两种方法检测56例ESCC中p53基因突变和蛋白的表达。结果56例ESCC组织中p53蛋白阳性表达率为61%(34/56),它与患者的年龄、性别和家族史无关(P>0.05),有和无淋巴结转移的阳性率分别为81%(17/21),49%(17/35);生存率<3年组和>3年组的p53阳性表达率为74%(17/23),46%(13/28),差异有统计学意义。PCR-SSCP检测p53基因突变率为77%(43/56),突变位于第4外显子者5例,第5外显子者23例,第6外显子者1例,第7外显子者4例,第8外显子者7例,有3例在内显子。p53基因突变/过度表达率为46%(26/56),两种方法检测的符合率为55%(31/56)。结论p53基因突变/过度表达在食管鳞癌发生、发展中可能发挥重要作用,可作为判断食管鳞癌患者预后的参考指标之一。
Objective To investigate p53 mutation and p53 protein accumulation in esophageal squarnous cell carcinoma （ESCC） and their correlation with clinicopathological feature and prognosis. Methods PCR-SSCP and immunohistochemistry technique were used to detect the p53 mutation and p53 protein expression. Results The general positive rate of p53 protein expression was 61% （34/56）. Its expression had no statistical signifiance in age, sex and family history （ P 〉 0.05）. The positive rate was 81% （ 17/21 ） in patients with lymph node metastasis vs 49 % （17/35） in ones without lymph node metastasis. The 3 year survival rate of patients with p53 protein expression was significantly lower than those without p53 protein expression. [46% （13/28） and 74% （17/23） respectively], p53 gene mutation was detected in 77% （43/56） of the same specimens by PCR-SSCP. In 5 cases, the mutation were located on exon 4,23 on exon 5,1 on exon 6,4 on exon 7,7 on exon 8 and 3 on intron respectively. The rate,of p53 gene mutation/overexpression was 46 % （26/56）. The concordance rate of immunohistochemistry and PCR-SSCP was 55 % （31/56）. Conclusion p53 gene mutation/overexpression may play an important role in the pathogeaaesis of esophageal squamous cell carcinoma and can be considered as a predicting marker of prognosis.
出处
《山西医药杂志》
CAS
2006年第5期399-400,共2页
Shanxi Medical Journal
