摘要
从文库的平均插入片段大小、文库的覆盖率、文库克隆的稳定性对中国美利奴细毛羊BAC文库进行了质量评价。统计结果表明,文库的平均插入片段大小约为133kb,非重组克隆占3.2%,文库在理论上约有8倍绵羊基因组覆盖率,从该文库中找到任意单拷贝序列的概率为99.93%。连续多次传代培养实验证实文库克隆的遗传稳定性好。
This paper evaluates the quality of BAC from those aspects： the average insert size of BAC, the depth of genome coverage, the stability of BAC clones. The statistics shows： the average insert size of BAC library is 133kb and the frequency of＇ clones without inserts is 3.2%. Thus, this library represents approximately 8-fold genome coverage. Theoretically, the probability to find any unique sequence in library screening is 99.93%. Tests on randomly selected clones demonstrated that they are very stable after serial growths.
出处
《石河子大学学报（自然科学版）》
CAS
2006年第1期102-104,共3页
Journal of Shihezi University(Natural Science)
