摘要
目的研究肿瘤坏死因子相关的凋亡诱导配体(TRAIL)受体在白血病细胞中的表达及其临床意义。方法应用RT-PCR方法对76例白血病患者的骨髓,包括15例急性淋巴细胞性白血病(急淋)、10例急性淋巴细胞性白血病缓解期(急淋缓解)、16例急性非淋巴细胞性白血病(急非淋)、11例急性非淋巴细胞性白血病缓解期(急非淋缓解)、12例慢性粒细胞性白血病急性变期(慢粒急变期)和12例慢性粒细胞性白血病慢性期(慢粒慢性期)以及25例正常人骨髓或外周血白细胞表面TRAIL受体的表达进行检测。结果急淋、急淋缓解、急非淋、急非淋缓解、慢粒急变期和慢粒慢性期患者骨髓的白细胞表面死亡受体(deadreceptor,DR)DR4和DR5表达高,而诱骗受体(decoyreceptor,DcR)DcR1和DcR2表达低,且DR4的表达高于DR5的表达;缓解期DR4和DR5的表达高于患者组;正常人骨髓或外周血白细胞中DR4和DR5表达低,而DcR1和DcR2表达高。结论TRAIL受体在不同类型的白血病细胞中的表达具有明显的差异性。
Objective TO investigate the expression of tumor necrosis factor related apeptosis induce ligand （TRAIL） receptors in human leukemia cells. Methods RT-PCR was used to detect the expression of TRAIL receptors on ALL （Acute Lymphoblastic Leukemia） cells of 15 cases ,ALL cells of 10 cases with complete remission （CR） ,AML （Acute Myeloid Leukemia） cells of 16 cases,AML cells of 11 cases with complete remission, CML （Chronic Myeloid Leukemia） cells of 12 cases with acute development,CML cells of 12 cases with chronic development and BMMNC or PBMNC of 25 normal persons. Resuits DR4 and DR5 were highly expressed in all leukemic cells,while the DcR1 and DcR2 were poorly expressed. The level of DR4 expression was significantly higher than that of DR5 expression in all leukemic ceils. The level of DR4 and DR5 expression in CR group were higher than that in patient group. In normal cells DR4 and DR5 were poorly expressed,while the DcR1 and DcR2 were highly expressed. Conclusions TRAIL receptors were, differently expressed in leukemic cells.
出处
《临床内科杂志》
CAS
2006年第5期348-350,共3页
Journal of Clinical Internal Medicine
基金
江苏省社会发展基金(BS2004008)
