摘要
目的评价本课题组构建的共表达FMDV衣壳蛋白前体P1-2A基因以及免疫辅助基因的重组鸡痘病毒vUTAL3CP1和重组DNA疫苗pVIRIL18P1在猪体内诱导特异性体液免疫和细胞免疫的能力。方法将上述2种疫苗采用4种不同的组合方式单独或联合给猪进行肌肉注射,用ELISA方法和中和实验检测猪产生的抗体水平,T淋巴细胞增殖反应、CTL反应以及T淋巴细胞亚类。结果这2种基因工程疫苗均能诱导猪产生特异性的体液及细胞免疫应答。其中单独免疫组vUTAL3CP1/vUTAL3CP1的效果最好,其诱导的抗体水平已接近于常规灭活疫苗,而细胞免疫水平则比后者高得多。联合免疫组中vUTAL3CP1/pVIRIL18P1可以诱导比vUTAL3CP1/vUTAL3CP1更高的CTL杀伤活性,但体液免疫水平略低。结论证实了这2种基因工程疫苗在猪体内均有良好的免疫原性。同时初步筛选出vUTAL3CP1/vUTAL3CP1和pVIRIL18P1/vUTAL3CP1组等较佳的免疫策略,为下一步的攻毒保护试验奠定了基础。
Objective To evaluate the immunogenicity of recombinant fowl-pox virus vUTAL3CP1 co-expressing P1-2A gene and 3C^pro gene of foot-and-mouth disease virus （FMDV） and DNA vaccine plasmid pVIRIL18P1 co-expressing P1-2A gene and pig IL-18 gene in pigs. Methods Pigs were inoculated intramuscularly with these 2 recombinant vaccines alone or in combination. The FMDV antibody were detected by ELISA and NT. T lymphoproliferation test, cytotoxic T lymphocyte response test, and T lymphocyte subpopulation were performed. Results Specific humoral and cellular immune responses could be induced in all recombinant vaccines inoculated groups. The vUTAL3CP1/ vUTAL3CP1 group had higher immunogenicity in 2 single immunizing groups. Comparing with inactivated vaccine, vUTAL3CP1/vUTAL3CP1 could induce the same FMDV antibody level and higher cellular immune responses in pigs. pVIRIL18P1/vUTAL3CP1 could induce higher CTL responses but lower antibody responses compared with vUTAL3CP1/vUTAL3CP1. Conclusion Both these 2 recombinant vaccines have good immunogenicity in pigs. The results suggest that vUTAL3CP1/vUTAL3CP1 and pVIRILI8P1/vUTAL3CP1 may be the most optimal vaccination strategies, which provides a support for further virus challenge tests.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第3期260-264,268,共6页
Immunological Journal
基金
国家"863"计划项目(2001AA213071)
吉林省科技厅重大项目(20040202-1)资助
关键词
口蹄疫病毒
重组鸡痘病毒
DNA疫苗
免疫原性
Foot-and-mouth disease virus
Recombinant fowl-pox viruses
DNA vaccine
Immunogenicity
