摘要
目的:模拟大鼠眼斜视手术脑组织中c-fos的变化,分析c-fos在脑组织不同部位的改变及可能作用机制。方法:实验于2003-07/09在潍坊医学院附属青州医院实验室完成。48只3月龄SD大鼠随机数字表法分为对照组(n=8)和手术组(n=40)。手术组进行右眼模拟眼斜视手术,随机两条动眼肌缩短术,并于术后2h,12h,1d,2d,5d5个时间点采用免疫组织化学染色分别测定各组大鼠脑组织外侧膝状体、嘴侧丘、视区c-fos的变化,c-fos免疫反应阳性神经元的细胞核呈棕黄色,胞浆和核仁均不染色。每只大鼠随机取脑组织切片10张,计数脑组织不同位置c-fos免疫反应阳性细胞数及阳性细胞总和。结果:纳入大鼠48只,均进入结果分析,无脱失。①在损伤眼同侧的外侧膝状体腹侧核、内侧核团和中间核可见c-fos阳性细胞,损伤后2h开始出现(20.58±9.36)个/切片,1d达到高峰(92.36±7.45)个/切片,2d减少(28.19±3.62)个/切片,5d仍有少量c-fos阳性细胞(12.88±3.41)个/切片。与对照组(8.04±3.06)个/切片比较,差异均有显著性意义(P<0.01或P<0.05)。②在损伤眼同侧的嘴侧丘、视区可见c-fos阳性细胞,损伤后2h开始出现(17.28±5.42)和(18.39±4.28)个/切片,1d达到高峰为(83.22±6.75)和(86.17±3.39)个/切片,2d减少为(25.18±4.33)和(29.52±3.27)个/切片,与对照组(9.35±2.98)及(13.72±3.51)个/切片比较,差异均有显著性意义(P<0.01)。5d仍有少量c-fos阳性细胞为(11.47±2.19)和(14.22±3.54)个/切片,与对照组接近,差异无显著性意义。结论:c-fos在脑外侧膝状体、嘴侧丘、视皮质参与损伤作用。脑外侧膝状体c-fos的变化在中枢调节作用中有中介作用,c-fos可能参与引起脑组织改变。
AIM： To simulate the changes of c-fos in brain tissues of rats received strabotomy, and analyze the changes of c-fos in different parts of brain tissues and its possible mechanism.
METHODS： The experiment was conducted in the Laboratory of Qingzhou Hospital Affiliated to Weifang Medical College from July to September 2003. Forty-eight SD rats, aged 3 months were randomly divided into control group （n=8） and operation group （n=40）. Rats in the operation group received simulated strabotomy on the right eye and shortening of two oculomotor muscles that randomly selected. Changes in pregeniculum, rostral cerebral ganglion and c-fos in visual cortex of brain tissues of rats in all groups were measured with immunohistochemieal stain respectively at 2 hours, 12 hours, 1 day, 2 days and 5 days after the strabotomy. The cellular nucleus of positive neurons in c-fos immune reaction were in buffy, and the endochylema and ehromatospherite were not stained. Ten pieces of brain-tissue sections were randomly selected from each rat, and the number of cofos positive cells in immune reaction of different parts of brain tissues and the total number of positive cells were calculated.
RESULTS： A total of 48 enrolled rats were involved in the analysis of results, and no one withdrew from the study.①Expression of c-fos positive cells could be seen＇in ventral nucleus, medial nucleus and intermedial nucleus of pregeniculum in the same lateral of injured eye, （20.58±9.36） of which existed on each section at 2 hours after injury, reached the peak （92.36±7.45） per section on the first day, decreased （28.19±3.62） per section on the second day, and there were little c-fos positive cells （12.88±3.41） per section on the fifth day. Compared with the control group （8.04±3.06） per section, the differences were remarkable （P 〈 0.01 or P 〈 0.05）. ②The c-fos positive cells could be seen in rostral cerebral ganglion and visual cortex of the same lateral in injured eye. At 2 hours after the injury, （17.28±5.42） and （18.39±4.28） represented in each section, and reached the peak （83.22±6.75） and （86.17±3.39） on the first day, decreased to （25.18±4.33） and （29.52±3.27） on the second day, compared with the control group （9.35±2.98） and （13.72±3.51） per section, the differences were significant （P 〈 0.01）. On the fifth day, there were still a small quantity of c-fos positive cells （11.47±2.19） and （14.22±3.54） on each section, which were similar to the control group, and differences were not significant.
CONCLUSION： The c-fos in pregeniculum, rostral cerebral ganglion and visual cortex takes part in the injury. The changes of c-fos in pregeniculum play an agent role in the regulation, and c-fos may contribute to the changes of brain tissue.
出处
《中国临床康复》
CSCD
北大核心
2006年第18期107-109,共3页
Chinese Journal of Clinical Rehabilitation
