摘要
利用途径工程的方法,将来源于克雷伯氏菌(Klebsiella pneumoniae)的甘油脱水酶基因dhaB和1,3-丙二醇氧化还原酶基因dhaT构建成多顺反子重组质粒pSE-dhaB-dhaT并在大肠杆菌JM 109中进行表达,在大肠杆菌中构建一条新的产1,3-丙二醇代谢途径。研究表明,重组菌株JM 109/pSE-dhaB-dhaT在微好氧条件下,尝试用廉价的乳糖为诱导物、维生素B12为辅酶,可以将甘油转化为1,3-丙二醇,产量达15.34 g/L,甘油转化率为35.7%,对低成本生产1,3-丙二醇作了有益的探索。
Based on the principle of the pathway engineering, a novel pathway of producing 1,3-propanediol was built in E. coll. The dhaB gene encoding glycerol dehydratase （DHAB） and the dhaT gene encoding 1, 3-propanediol oxidoreductase （DHAT） were cloned from Klebsiella pneumoniae and then inserted into expression vector pSE380 to obtain recombinant polycistron plasmid pSE-dhaB-dhaT. This polycistron was transformed into E.coli JM109. The result showed that the recombinant microorganism JM109/pSE-dhaB-dhaT could convert glycerol to 1,3-propanediol directly under the induced conditions of lactose and vitamin B12 in the fermentor,and the maximal concentration of 1,3-propanediol was 15.34 g/L at 60 h, conversion rate of glycerol was 35.7 % under the microaerobic condition.
出处
《生物加工过程》
CAS
CSCD
2006年第1期39-43,共5页
Chinese Journal of Bioprocess Engineering
基金
国家"863"高科技资助项目(2003AA001039)
关键词
1
3-丙二醇
重组菌
发酵条件
1,3-propanediol
recombinant microorganism
ferment conditions
