摘要
构建了两种新的重组人泡沫病毒载体pGPSNI-hGAD67和pGPSNI-hGAD65,分别携带人谷氨酸脱羧酶(GAD)的两种同工酶GAD65和GAD67基因,并研究其在帕金森病(PD)模型鼠丘脑底核中的表达及对帕金森病的治疗作用.RT-PCR结果表明,人泡沫病毒载体成功地介导了GAD65基因和GAD67基因在PD模型鼠丘脑底核中有效表达.动物行为检测表明,GAD65基因导入组PD模型鼠行为与对照组相比得到明显的改善(n=12,p<0.01).
In this study, two novel replication-defective human foamy virus （HFV） vectors plasmids pGPSNI-hGAD67 and pGPSNI-hGAD65 were constructed and contain two transcription units： one enco ding the neomycine phosphotransferase gene and the other encoding a single human glutamic acid decarboxylase （GAD） isoforms （hGAD67 or hGAD65）. Furthermore, the GAD gene mediated by HFV was trans duced in excitatory glutamatergic neurons of the subthalamic nucleus （STN） of Parkinson＇s Disease （PD） rats, so that the gene transcription and expression and the theraputic effect on PD rats models was observed. The results of RT PCR suggest that effective foreign genes were expressed in the STN of PD ra, ts by successful GAD65 or GAD67 gene transduction through a HFV vector mediating GAD gene. To determine animal behavior, we found that PD rats received C-AD65 gene treatment revealed decreasing rotation （n=12, p〈0.01）.
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2006年第2期202-206,共5页
Journal of Wuhan University:Natural Science Edition
基金
国家自然科学基金资助项目(30200083)
湖北省科技攻关计划资助项目(2002AA304B09)
湖北省杰出青年基金资助项目(2004ABB001)
武汉大学引进人才科研启动基金项目(502-276001)
关键词
人泡沫病毒载体
谷氨酸脱羧酶
帕金森病
基因治疗
human foamy virus （HFV） vectors
glutamic acid decarboxylase （GAD）
Parkinson＇s Disease （PD）
gene therapy
