摘要
目的:探讨大豆异黄酮对卵巢切除大鼠血脂和低密度脂蛋白受体(LDLR)mRNA表达的影响。方法:随机将大鼠分为假手术组、卵巢切除组、卵巢切除+吉非罗齐组和卵巢切除+大豆异黄酮组,去除双侧卵巢2周后治疗3个月,股动脉放血处死大鼠,取血清用于血脂测定。取大鼠肝脏组织,用反转录PCR(RT-PCR)检测低密度脂蛋白受体(LDLR)mRNA表达。结果:与卵巢切除组比较,大豆异黄酮治疗组血清TG、LDL-C和OX-LDL明显降低,HDL-C显著升高。大豆异黄酮治疗组LDLR mRNA表达水平明显升高,与卵巢切除和吉非罗齐组大鼠比较差异均有显著性。结论:卵巢切除后,大鼠肝脏组织低密度脂蛋白受体基因表达水平下调。大豆异黄酮可能通过转录水平调节升高肝脏组织LDLR mRNA表达水平,从而起到降低血清LDL的作用。
Objective ： To investigate the effect of soybean isoflavone on blood lipid and the expression of LDLR mRNA in ovariectomied rats. Methods ： Forty eight Sprague-Dawley rats were divided randomly into four groups ： Sham group （sham） , OVX group （OVX）, OVX ＋ gemiflbrozil （G） group （OVX ＋ G） and OVX ＋ isoflavone （ISO） group （OVX_ISO）. in which the rats were treated with G or ISO for three months starting from two weeks after both sides of rat＇s ovarietomy. Blood sample were taken out for determination of blood lipid. The liver tissue were taken out quickly, Isothiocyanate guanidine-phenol-chloroform was used to extract the total RNA from the liver and RT-PCR was used to detect the expression of LDLR mRNA. Results： Compared with the OVX group, contents of TG, LDL-C and OX-LDL in serum in OVX ＋ ISO group decreased remarkably, and the contents of HDL-C increased. LDLR mRNA in OVX ＋ ISO group increased distinctively compared with OVX group and OVX ＋ G group. Conclusion ： The level of mRNA of LDLR in liver decrea.s after ovariotormy and the soybean isoflavone may increase it by regulating the expression level of LDLR through transcription.
出处
《中药材》
CAS
CSCD
北大核心
2006年第4期349-351,共3页
Journal of Chinese Medicinal Materials
基金
北京联合大学中青年骨干教师资助项目
关键词
大豆异黄酮
卵巢切除
血脂
低密度脂蛋白受体
Soybean isoflavone
Ovariotomy
Blood lipid
Low density lipoprotein receptor（LDLR）
