摘要
目的：探讨建立成年大鼠心肌细胞钙超载模型的方法。方法：Langendorff灌流，分离钙耐受成年大鼠心肌细胞，采用不同浓度钙离子导入剂-伊屋诺霉素（ionomycin）作用心肌细胞1小时，用荧光分光光度计测定心肌细胞内的游离钙浓度。结果：1．分离的活性心肌细胞比率约70～80％，杆状、横纹清晰。2．实验组1（ionomycin0．5μmol／L）心肌细胞[Ca^2＋]1明显高于对照组（282．07±25．19 vs 115±21．21nmol／L，P〈0．05）；实验组2（ionomycin 1．0μmol／L）心肌细胞[Ca^2＋]1（376．35±29．44）显著高于正常对照组（P〈0．05）。结论：通过本方法可以建立成年大鼠心肌细胞钙超载模型。
Objective： To develop the calcium overload model with adult rat ventricular cardiomyoeytes.Methods： Calcium tolerant cardiomyocytes were isolated by Langendorff perfusion. Then, cardiomyocytes were exposed to ionomycin for 1 hour, and cytosolic calcium was measured with spectrofluorometer. Results： Viability of freshly isolated adult rat ventricular cardiomyocytes, which were rod- shaped with clear cross- striations, was 60% - 80% . Cytosolic calcium of cardiomyocytes stimulated by 0.5μmol/L or 1.0μmol/L ionomycin increased significantly by 145 % （ P 〈 0.05, vs control） and 226 % （ P 〈 0.05, vs control） respectively, Conclusion： Calcium overload model of adult rat ventricular cardiomyocytes can be built by ionomycin.
出处
《华西医学》
CAS
2006年第2期308-309,共2页
West China Medical Journal
关键词
大鼠
心肌细胞
钙超载模型
Rat
Cardiomyocytes
calcium overload model
