摘要
Cereal aleurone has been established as a model system to investigate giberrellin （GA） and absclslc acid （ABA） responses. Using Barley 1 GeneChip, we examined the mRNA accumulation of over 22 000 genes In de-embryonated barley aleurone treated with GA and ABA. We observed that 1 328 genes had more than a threefold change In response to GA treatment, whereas 206 genes had a more than threefold change in response to ABA treatment. Interestingly, approximately 2.5-fold more genes were up-regulated than downregulated by ABA. Eighty-three genes were differentially regulated by both GA and ABA. Most of the genes were subject to antagonistic regulation by ABA and GA, particularly for genes related to seed maturation and germination, such as genes encoding late embryogenesis abundant proteins and storage mobilization enzymes. This supports the antagonistic roles of GA and ABA in seed maturation and seed germination. Interestingly, we observed that a significant percentage of the genes were coordinately regulated by both GA and ABA. Some GA-responsive genes encoded proteins involved in ethylene, Jasmonate, brasslnosterold and auxin metabolic and signaling transducUon pathways, suggesting their potential Interaction with the GA response. We also identified a group of transcription factor genes, such as MYB and Homeobox genes, that were differentially regulated by GA. In addition, a number of GA- and/or ABA-responsive genes encoded components potentially Involved in GA and ABA signal transducUon pathway. Overall, the present study provides a comprehensive and global view of transcript expression accompanying the GA and ABA response in barley aleurone and Identifies a group of genes with potential regulatory functions in GA- and ABA-slgnallng pathways for future functional validation.
Cereal aleurone has been established as a model system to investigate giberrellin （GA） and absclslc acid （ABA） responses. Using Barley 1 GeneChip, we examined the mRNA accumulation of over 22 000 genes In de-embryonated barley aleurone treated with GA and ABA. We observed that 1 328 genes had more than a threefold change In response to GA treatment, whereas 206 genes had a more than threefold change in response to ABA treatment. Interestingly, approximately 2.5-fold more genes were up-regulated than downregulated by ABA. Eighty-three genes were differentially regulated by both GA and ABA. Most of the genes were subject to antagonistic regulation by ABA and GA, particularly for genes related to seed maturation and germination, such as genes encoding late embryogenesis abundant proteins and storage mobilization enzymes. This supports the antagonistic roles of GA and ABA in seed maturation and seed germination. Interestingly, we observed that a significant percentage of the genes were coordinately regulated by both GA and ABA. Some GA-responsive genes encoded proteins involved in ethylene, Jasmonate, brasslnosterold and auxin metabolic and signaling transducUon pathways, suggesting their potential Interaction with the GA response. We also identified a group of transcription factor genes, such as MYB and Homeobox genes, that were differentially regulated by GA. In addition, a number of GA- and/or ABA-responsive genes encoded components potentially Involved in GA and ABA signal transducUon pathway. Overall, the present study provides a comprehensive and global view of transcript expression accompanying the GA and ABA response in barley aleurone and Identifies a group of genes with potential regulatory functions in GA- and ABA-slgnallng pathways for future functional validation.
基金
Supported by the US Department of Agriculture, Agricultural Research Service and the US Barley Genome Project. Publication of this paper is supported by the National Natural Science Foundation of China （30424813） and Science Publication Foundation of the Chinese Academy of Sciences.
