摘要
29日龄肉鸡腹腔接种0.5mL大肠杆菌肉汤悬浮液(O2标准菌株,2.1×109CFU/mL)建立了肉鸡大肠杆菌病模型,对其病理指标进行了观测与分析。结果表明:感染1h后肉鸡表现出发病症状,10h后发病率100%;试验期间死亡率55%,死亡时间在19~65h,扑杀感染鸡的脏器病变率为心包炎55%、肝周炎85%、气囊炎65%;感染后1~3d,肝脏大肠杆菌阳性率为100%,第7天为80%。感染后1~3d的体温极显著高于对照组(P<0.01)。感染后1~7d肉鸡的股动脉收缩压、舒张压和平均股动脉压极显著低于(P<0.01)对照组,分别平均下降了26.25%、27.72%、27.12%。感染后第7天肉鸡红细胞压积显著低于(P<0.05)对照组。
The 29-days-old broilers were inoculated intraperitoneally with 0.5 mL nutrient broth suspension of E.coli (O2 standard strains,2.1×10^9 CFU/mL)to establish model of eolibaeillosis in broilers,and its pathologieal indexes were examined and analysed.Infected broilers showed elinieal signs 1 hour after infection,and ineidenee of eolibaeillosis in broiler were 100% 10 hours after infection.The mortality of infected ehiekens was 55% in a week after infection,the time of birds death was 19 to 65 hours,and the ineidenees of eardioperieardifis,pefihepatitis and air sac inflammation of the killed birds were 55% ,85% and 65% respectively.The positive rate of E.coli in the liver was 100% in the first three days after infeetion and 80% on 7th day after infection.Body temperature of infected broilers was signifieanfly higher than that of the eontrol group in the first three days after infection (P〈0.01),and femoral artery systolie/diastolic pressure and mean femoral artery pressure were signifieanfly lower (26.25%,27.72%,27.12%)than those of the eontrol group in a week after infection (P〈0.01),and packed eell volume was significantly lower than that of the eontrol group on 7th day after infection (P〈0.05).
出处
《中国家禽》
北大核心
2006年第8期12-15,共4页
China Poultry
关键词
腹腔接种
肉鸡
大肠杆菌病模型
病理指标
intraperitoneal inoculation
broiler
model of colibacillosis
pathologieal index