摘要
目的研究3p、9p微卫星DNA异常在肺癌早期诊断中的价值。方法用PCR—银染法从外周血检测原发性肺癌病人、肺部良性病人及正常人3p14、3p21、9p21上的三个微卫星位点（D3S1228、D3S1029、D9S171）的异常表现。结果肺癌病人血清中DNA含量多于良性病人及正常人。肺癌组各微卫星位点的MSI或LOH异常的阳性率在43。50％之间（n=105）．以D3S1029最高。达到49．6％。三个位点中至少一个位点出现微卫星异常为76．2％，其中有45．7％呈多位点的改变。与肺良性病变组（n=97）及健康对照组（n=7）均有显著差异（P〈0．05）。在肺癌组中，各个微卫星位点的异常表现与肺癌临床分期和临床病理类型之间无明显差异（P〉0．05）。结论3p、9p微卫星DNA异常和肺癌分期无关，可以作为一项肺癌早期基因检测的新途径。不同微卫星位点出现异常表现的具体形式有所不同。多位点联合检测可以提高诊断的敏感性和特异性。
Objeclive To study the value of early diagnosis of chromosome 3p and 9p microsatellite DNA abnormity in lung cancer.Methods Using PCR and sliver-dye techniques ,three microsatellite sites （D3S1228.D3S1029,D9S171）on 3p14,3p21, 9p21 DNA abnormity in the whole blood samples from primary lung cancer patients,lung benign disease patients and healthy controls were detected.Results The content of DNA in serum in lung cancer patients was more than that of lung benign disease patients and healthy controls. The positive rate of MSI or LOH at single locus was between 43% and 50%,the site D3S1029 was the highest and reached 49.6%, at lease 1 site accurs to change in 3 sites was 76.2% in lung cancer patients, many sites appear to change was 45.7% ,which were significant difference than benign lung disease group and healthy controls （P〈0.05）.The microsatellite DNA abnormity showed without marked difference between the clinic stage and pathologic type in lung cancer patients （P〉0.05）.Conclusion Chromosome 3p and 9p microsatellite DNA abnormity has no relationship to clinic stage of lung cancer, it may use as a new approach to early detection of lung cancer in gene level. Microsatellite DNA abnormity concrete form is various in different sites, ninny sites simultaneous detection may improve the sensitivity and specificity.
出处
《结核病与胸部肿瘤》
2006年第2期85-92,共8页
Tuberculosis and Thoracic Tumor
关键词
微卫星
肺肿瘤
早期诊断
Microsatellite Lung neoplasms Early diagnosis
