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胃癌DNA甲基化差异片段的筛选与分析

Isolation and analysis of the differentially methylated DNA sequences of gastric cancer
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摘要 目的筛选和分析胃癌和正常胃组织间DNA甲基化差异片段。方法通过甲基化敏感性代表性差异分析法(methylation-sensitive-representationaldifferenceanalysis,MS-RDA),筛选胃癌和正常胃组织间DNA甲基化差异片段,经克隆、测序后进行生物信息学分析。结果获得3个甲基化差异片段CRS1308、CRS1309、CRS1310序列。其中CRS1309和CRS1310已被GenBank收录,登陆号分别为AY887106和AY887107。CRS1309序列与LOC440683基因、LOC440887基因的3'端、DRD5基因均有很高的相似性;CRS1310序列与1999年MinoruToyota在人类结肠癌中分离出的核糖体RNA上的甲基化差异性CpG岛有很高的相似性。结论胃癌和正常胃组织间DNA甲基化存在差异,LOC440683、LOC440887、DRD5基因的异常甲基化可能与胃癌的发生相关,胃癌中CRS1310序列所在核糖体RNA上的CpG岛易发生甲基化。 Objective：To isolate and analyze the differentially methylated DNA sequences between gastric cancer and normal gastric mucosa. Methods：The differentially methylated DNA sequences between gastric cancer and normal gastric mucosa were isolated by Methylation- sensitive -representational difference analysis（ MS- RDA）. Similarities between the separated fragments and the human genmic DNA were analazed with BLAST program system. Results： Three differentialy methylated DNA sequences, CRS1308, CRS1309, CRS1310, were obtained. CRS1309 and CRS1310 were accepted by GenBank. Studies indicated that CRS1309 was highly similar with three genes LOC440683, LOC440887, DRD5 ;CRS1310 was concerned with a CpG island , in ribosomal RNA, isolated from colorectal cancer by Minoru Toyota in 1999. Conclusion：The methylation degree between gastric cancer and normal gastric mucosa is different. The aberrant methylation of LOC440683, LOCA40887, DRD5 may be concerned with gastric cancer and CRS1310 is prone to be methylated in gastric cancer.
作者 王珣 廖爱军 苏琦
机构地区 南华大学附一医院消化内科 南华大学肿瘤研究所
出处 《现代肿瘤医学》 CAS 2006年第5期581-584,共4页 Journal of Modern Oncology
基金 湖南省教育厅科学研究项目(项目编号:99C195)
关键词 胃癌 DNA甲基化 差异片段 筛选 分析 gastric cancer DNA methylation differentially DNA sequences .
分类号 R735.2 [医药卫生—肿瘤]
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参考文献9

  • 1Toyota M,Itoh F,Imai K.DNA methylation and gastrointestinal malignancies; functional consequences and clinical implications[J].J Gastroenterol,2000,35:727 ～ 734.
  • 2Ushijima T,Morimura K,Hosoya Y,et al.Establishment of methylation-sensitive-reprsentational difference analysis and isolation of Hypo-and hypermethylated genomic fragments in mouse liver tumors[J].Proc Natl Acad Sci USA,1997,94:2284 ～ 2289.
  • 3Minoru Toyota,Coty Ho,Nita Ahuja,et al.Identification of differentially methylated sequences in colorectal cancer by methylated c pG island amplification1[J].Cancer Research,1999,59:2307～2312.
  • 4Hou P,Ji M,Ge C,et al.Detection of methylation of human p16 (Ink4a) gene 5'-CpG islands by electrochemical method coupled with linker-PCR[J].Nucleic Acids Res,2003,31 (16):e92.
  • 5Tan YC,Chow VT.Novel human HALR (MLL3) gene encodes a protein homologous to ALR and to ALL-1 involved in leukemia,and maps to chromosome 7q36 associated with leukemia and developmental defects[J].Cancer,2001,25 (5):454 ～ 469.
  • 6Ruault M,Brun ME,Ventura M,et al.MLL3,a new human member of the TRX/MLL gene family,maps to 7q36,a chromosome region frequently deleted in myeloid leukaemia[J].Gene,2002,284(1 ～ 2):73 ～ 81.
  • 7周俊,段丽萍,叶嗣懋,韩亚京,白若韵,林三仁.多巴胺受体亚型D_4和D_5在人胃、十二指肠的定位及表达[J].中华消化杂志,2003,23(7):403-406. 被引量：9
  • 8Mezey E,Eisenhofer G.Non-neuronal dopamine in the gastrointestinal system[J].Clin Exp Pharmacol Physiol Suppl,1999,26:S14 ～ 22.
  • 9向华,侯晓华.多巴胺及其受体与胃黏膜保护[J].世界华人消化杂志,2003,11(12):1997-1999. 被引量：1

二级参考文献28

  • 1Hunyady B, Hoffman BJ, Eisenhofer G, Hansson SR, Mezey E.Dopaminergic characteristics of isolated parietal cells from rats. J Physiol Paris 1997;91:247-256
  • 2Monsma FJ Jr, Mahan LC, McVittie LD, Gerfen CR, Sibley DR.Molecular cloning and expression of a D1 dopamine receptor linked to adenylyl cyclase activation. Proc Natl Acad Sci USA 1990;87:6723-6727
  • 3Monsma FJ Jr, McVittie LD, Gerfen CR, Mahan LC, Sibley DR.Multiple D2 dopamine receptors produced by alternative RNA splicing. Nature 1989;342:926-929
  • 4Van Tol HH, Bunzow JR, Guan HC, Sunahara RK, Seeman P,Niznik HB, Civelli O. Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine. Nature 1991;350:610-614
  • 5Sokoloff P, Giros B, Martres MP, Bouthenet ML, Schwartz JC.Molecular cloning and characterization of a novel dopamine receptor(D3) as a target for neuroleptics. Nature 1990;347:146-151
  • 6Sunahara RK, Guan HC, O'Dowd BF, Seeman P, Laurier LG,Ng G, George SR, Torchia J, Van Tol HH, Niznik HB. Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1. Nature 1991;350:614-619
  • 7Glavin GB, Szabo S. Dopamine in gastrointestinal disease.Dig Dis Sci 1990;35:1153-1155.
  • 8Glavin G. Activity of selective dopamine DA1 and DA2 agonists and antagonists on experimental gastric lesions and gastric acid secretion. J Pharmacol Exp Ther 1989;251:726-730.
  • 9Shyu JC, Chu CY, Duh SL, Liu JY, Chang CL. Nucleus accumbens dopaminergic mediation of protection againstgastric mucosal ulcerations by cold-restraint stress in the male hyperprolactinemic rats. Chin J Physiol 1997; 40:37-43
  • 10Kauffman GL. Stress, the brain, and the gastric mucosa. Am J Surg 1997;174:271-275

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现代肿瘤医学
2006年 第5期

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