摘要
杯菊(Cyathocline purpurea)是云南民间民族药,全株用于治疗各种炎症和肺结核.采用L1210细胞系对杯菊的粗提物、分步萃取物的成分进行了细胞毒试验;用柱层色谱法同步对活性组分进行化学成分分离纯化;通过理化数据测定及波谱分析鉴定单体结构.杯菊的氯仿及乙酸乙酯萃取物具有细胞毒活性,其IC50分别为3.5和2.8μg/mL.从活性组分中分离出3种成分,分别鉴定为Santam arin(1)、9β-乙酰基广木香内酯(9-βacetoxycostunolide,2)和9β-乙酰基小白菊内酯(9-βacetoxypathenolide,3),其IC50分别为0.41,0.89,0.59μg/mL.这三个化合物都有较强的抗癌活性,其中化合物3为新化合物.
Cyathocline purpurea （ Buch-Ham ex D Don） O Ktze is a folk drug used to treat inflammations and pulmonary tuberculosis in Yunnan Province. Cytotoxicity screening of extracts and its fractions from C. pur- purea was carried out by L1210 cell. Isolation of chemical constituents by column chromatography was undertaken in step with cytotoxicity test. The structures of constituents were identified by spectroscopic and chemical methods. The chloroform and ethyl acetate extracts possess cytotoxic activity with IC50＇s being 3.5 and 2.8 μg/mL, respectively. Three sesquiterpene lactones were established as Santamarin （compound 1 ）, 9β-acetoxycostunolide（ compound 2） and 9β-acetoxypathenolide （compound 3 ）. Their IC50 were 0. 41, 0. 89, 0. 59 μg/mL by L1210 cell respectively. The three constituents were obtained for the first time from this plant. They are all anticancer active constituents. The last one is a new compound.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第5期859-862,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:29962004)
云南省国际合作项目(批准号:2001GH21)资助
