摘要
AIM： To assess subcellular localization of KL-6 mucin and its clinicopathological significance in colorectal carcinoma as well as metastatic lymph node and liver tissues. METHODS： Colorectal carcinoma tissues as well as metastatic lymph node and liver tissues were collected from 82 patients who underwent colorectomy or hepatectomy. Tissues were subjected to immunohistochemical analysis using KL-6 antibody. RESULTS： Of the 82 colorectal carcinoma patients, 6 showed no staining, 29 showed positive staining only in the apical membrane, and 47 showed positive staining in the circumferential membrane and/or cytoplasm. Positive staining was not observed in non-cancerous colorectal epithelial cells surrounding the tumor tissues. The five-year survival rate was significantly lower in cases showing positive staining in the circumferential membrane and/or cytoplasm （63.0%） than those showing positive staining only in the apical membrane （85.7%） and those showing no staining （100%）. Statistical analysis between clinicopathological factors and subcellular localization of KL-6 mucin showed that KL-6 localization in the circumferential membrane and/or cytoplasm was significantly associated with the presence of venous invasion （P = 0.0003）, lymphatic invasion （P 〈 0.0001）, lymph node metastasis （P〈0.0001）, liver metastasis （P = 0.058）, and advanced histological stage （P〈 0.0001）. Positive staining was observed in all metastatic lesions tested as well as in the primary colorectal carcinoma tissues. CONCLUSION： The subcellular staining pattern of KL-6 in colorectal adenocarcinoma may be an important indicator for unfavorable behaviors such as lymph node and liver metastasis, as well as for the prognosis of patients.
瞄准：估计在颜色代替 KL-6 粘蛋白和它的 clinicopathological 意义的细胞的本地化表面的癌以及变形淋巴节点和肝纸巾。方法：Colorectal 癌纸巾和肝纸巾从经历了 colorectomy 或肝切除术的 82 个病人被收集。纸巾受到免疫组织化学的分析使用 KL-6 抗体。结果：82 颜色表面的癌病人， 6 没显示出染色， 29 显示出仅仅在顶端的膜的积极染色，并且 47 显示出在环绕的膜或细胞质的积极染色。积极染色没在非癌的颜色被观察包围肿瘤纸巾的表面的上皮细胞。五年的幸存率在显示出比显示出仅仅在顶端的膜(85.7%) 的积极染色的那些和不显示出染色的那些(100%) 的在环绕的膜或细胞质(63.0%) 的积极染色的情况中是显著地更低的。在 KL-6 粘蛋白的细胞的本地化在环绕的膜或细胞质看了那 KL-6 本地化的 clinicopathological 因素和代用品之间的统计分析显著地与静脉的侵略的存在被联系( P = 0.0003 )，淋巴的侵略( P<0.0001 )，淋巴节点转移( P<0.0001 )，肝转移( P = 0.058 )，并且先进组织学的舞台( P<0.0001 )。积极染色在测试的所有变形损害以及在三原色被观察表面的癌纸巾。结论：潜水艇在颜色的 KL-6 的细胞的染色模式表面的腺癌可以是为象淋巴节点和肝转移那样的相反的行为，以及为病人的预后的重要指示物。
基金
Supported by Grants-in-aid from the Ministry of Education,Science,Sports and Culture of Japan and a grant for Hi-Tech Research from Tokai University
