摘要
目的:探讨卵巢癌缺失2(DOC2)基因对宫颈癌Si-Ha细胞系生长的抑制作用.方法:采用基因转染技术,将含有全长DOC2cDNA的真核重组表达质粒和空载体质粒(pcD-NA3.1)转染到人宫颈癌SiHa细胞系(无DOC2基因的表达)中,了解其对细胞增殖能力及细胞周期的影响.结果:转染DOC2基因的宫颈癌细胞生长受到抑制(P<0.05),其在软琼脂克隆形成能力明显降低(P<0.05).DOC2有使G1期细胞比例增高、S期细胞比例下降的趋势,但SiHa细胞和SiHa-pcD-NA3.1细胞之间无显著差异.结论:DOC2基因能抑制宫颈癌细胞系的增殖.
AIM： To explore the inhibitory effect of DOC2 gene on the growth of human cervical cancer cell line SiHa. METHODS ： Recombinant eukaryotic expression vector pcDNA3.1-p93 （containing exogenous human DOC2cDNA） and vector pcDNA3. 1 （with neomycin resistance gene only ） were transfected into human cervical cancer SiHa cells （ no DOC2 gene expression） with lipofectamine respectively. The growth rate, cell cycle and colony formation rate of transfected cells were observed by MTT assay and FCM assay respectively. RESULTS： The growth of SiHa transfected with DOC2 gene was markedly suppressed （P 〈 0.05 ）. Colony formation rate in soft agar was also decreased significantly （P 〈 0.05 ）. Cell cycle analysis showed that the percentage of cells in G1 phase of SiHa-p93 cells was significiantly increased while that of cells in S phase was decreased. The percentage of cells in the different phases of the cycle did not significantly vary between the SiHa and SiHa-pcDNA-3. 1. CONCLUSION： DOC2 gene can inhibit the proliferation and DNA synthesis of human cervical cancer.
出处
《第四军医大学学报》
北大核心
2006年第8期741-743,共3页
Journal of the Fourth Military Medical University
基金
陕西省自然科学基金资助项目(2003C2023)
