摘要
目的:观察食管断端正常鳞状上皮与鳞癌组织中血管紧张素Ⅱ及血管紧张素Ⅱ受体1的表达。方法:实验于2005-07/10在河南省肿瘤重点实验室完成。采用免疫组织化学SP法检测49例食管断端正常鳞状上皮及53例食管鳞癌组织中血管紧张素Ⅱ及血管紧张素Ⅱ受体1的表达。在高倍镜下选取阳性细胞最多的区域,记录5个高倍视野中血管紧张素Ⅱ、血管紧张素Ⅱ受体1阳性细胞的百分比,计算其平均值。阳性细胞<10%为穴-雪;10%～30%为穴+雪;30%～50%为穴誒雪;>50%为穴誔雪。结果:血管紧张素Ⅱ和血管紧张素Ⅱ受体1定位于胞浆和胞膜。血管紧张素Ⅱ主要为弥漫性表达,在正常鳞状上皮和鳞癌组织中的阳性表达率分别是51.02%(25/49)和86.79%(46/53)。血管紧张素Ⅱ受体1在46.94%(23/49)的正常鳞状上皮的扁平细胞层和棘层细胞表达,在77.36%(41/53)的鳞癌组织中主要呈弥漫性表达,部分癌巢可见“阴性外围”表达模式。血管紧张素Ⅱ、血管紧张素Ⅱ受体1在鳞癌组织中的表达均显著高于正常鳞状上皮穴均P<0.01雪。血管紧张素Ⅱ在各级鳞癌组织中的表达差异无显著性穴P>0.05雪;血管紧张素Ⅱ受体1在I级鳞癌组织中的表达显著高于Ⅱ、Ⅲ级鳞癌组织穴分别P<0.05,P<0.01雪,在Ⅱ、Ⅲ级鳞癌组织中的表达差异无显著性穴P>0.05雪。血管紧张素Ⅱ、血管紧张素Ⅱ受体1在鳞癌侵及食管深层和浅层、有淋巴结转移和无淋巴结转移的表达差异均无显著性(P>0.05)。结论:血管紧张素Ⅱ受体1可能与食管鳞癌的分化有关,血管紧张素Ⅱ及其血管紧张素Ⅱ受体1可能参与食管鳞癌的发生发展,但与食管鳞癌的浸润和淋巴结转移无明显关系。
AIM： To observe the expressions of angiotensin Ⅱ （Ang Ⅱ ） and Ang Ⅱ type 1 receptor （AT1R） in the normal squamous epithelium and esophageal squamous cell carcinoma （ESCC）.
METHODS： The experiment was undertaken in the Henan Key Laboratory for Tumor Pathology from July to October 2005. SP immunohistochemical method was performed to detect the expressions of Ang Ⅱ and AT1R in the ESCC tissues of 53 cases and normal esophageal squamous epithelium of 49 cases. The percentages and average value of Ang Ⅱ and AT1R positive cells were estimated in five high-power fields which comprised the most positive cells, and graded into one of four categories： （-）,〈10%; （＋）,10%-30%; （＋＋）,30%-50%; （＋＋＋）,〉50%.
RESULTS： Ang Ⅱ and AT1R were localized in the cytoplasm and membrane of cells, and Ang Ⅱ .was mostly expressed diffusely. The rates of positive expression of Ang Ⅱ in normal esophageal squamous epithelium and ESCC tissues were 51.02% （25/49） and 86.79% （46/53） respectively. AT1R were expressed in the stratum spinosum and pinacocyte layer of 46.94% （23/49） normal esophageal squamous epithelia, and diffusely expressed in 77.36% （41/53） ESCC tissues, with the ＂negative periphery＂ pattern was observed in some cancer nests. The expressions of Ang Ⅱ and AT1R were significantly higher in ESCC tissues than in normal esophageal squamous epithelia （P 〈 0.01）. There was no significant difference in the expressions of Ang Ⅱ in ESCC tissues of different grades （P 〉 0.05）; The expression of AT1R was significantly higher in grade Ⅰ than in grade Ⅱ and grade Ⅲ of ESCC tissues （P 〈 0.05, P 〈 0.01）, with no significant difference between grade Ⅱ and grade Ⅲ of ESCC tissues （P 〉 0.05）. Differences were also insignificant in the expressions of Ang Ⅱ and AT1R not only between the superficial layer and deep layer of esophagus invaded by ESCC but also between the lymph node metastasis group and non-lymph node metastasis group （P 〉 0.05）.
CONCLUSION： AT1R may be associated with the differentiation of ESCC. Ang Ⅱ and AT1R are possibly involved in the occurrence and development of ESCC, but unrelated with the infiltration and lymph node metastasis of ESCC.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第20期101-103,i0004,共4页
Chinese Journal of Clinical Rehabilitation
