摘要
用日龄2天的SD大鼠大脑皮层进行分散神经细胞原代培养，通过氰化物阻断细胞呼吸链的方法，模拟了大脑皮层神经细胞的急性缺氧损害模型。研究了氟桂嗪对缺氧损害细胞的作用，观察到缺氧细胞恢复过程中3H─TdR和3H─UR的参入量显著高于对照组；氟桂嗪保护组细胞3H─TdR和3H一UR参入量与缺氧组相比显著降低；氟桂嗪也可使正常皮层细胞3H─TdR和3H─UR参入量减少。
Primary cultures of rat cerebral cortical neurons were prepared from cortices of 2 days Sprague──Dawley rats and made an attempt to imitate the hypoxic neurons injury for testing neuroprotective effect of flunarizine. Hypoxia was induced hy adding lmmol/L sodium cyanide to the incubation medium of the neurons. It was found that hypoxic group had significantly higher incorporation of 3H－TdR and 3H－UR than control group, while flunarizine can decrease the incorporation of 3H－TdR and 3H－UR. In addition, flunarizine can decrease the incorporation of 3H－TDR and 3H－UR in normal cultured neurons. The results showed that flunarizine can regulate the synthesis of DNA amd RNA
出处
《新生儿科杂志》
1996年第1期22-24,共3页
The Journal of Neonatology
