摘要
目的探讨增殖细胞核抗原(PCNA)和p53蛋白在大肠癌中的表达及临床意义。方法应用免疫组化S-P方法检测60例大肠腺癌中的PCNA及p53蛋白的表达。结果大肠癌中p53蛋白阳性表达率为63.3%,PCNA增殖指数为(78.2±24.5)%,p53蛋白表达阳性者其细胞增殖活性为(83.1±18.6)%,明显高于p53蛋白阴性组(61.2±11.3)%(P<0.01)。p53蛋白阳性表达率与PCNA增殖指数随着大肠癌病理分级的上升而增加,且与预后呈负相关。结论同时检测p53蛋白、PCNA对大肠癌的诊断、病理分级及预后的评估有重要的指导意义。
Objective To study the expression and clinical significance of proliferating cell nuclear antigen （PCNA） and p53 protein in colorectal carcinoma. Methods The expression of p53 protein and PCNA was investigated in 60 cases of colorectal carcinoma using S-P immunohistochemical method. Results The positive expression of p53 was 63.3%, PCNA proliferative index was 78.2 ± 24.5%. The cell proliferative index in p53-positive cases was 83.1% ± 18.6%, significantly higher than that in p53-negative cases （61.2 ± 11.7）% （P 〈0.01 ）. Both the positive rates of p53 expression and PCNA proliferative index were increased with rising of pathological grades, but they showed a negative correlation with prognosis. Conclusion It may be useful to examine p53 protein and PCNA for diagnosis, pathological grades and prognosis of colorectal carcinoma.
出处
《中国肿瘤临床与康复》
2006年第2期111-113,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
