摘要
目的利用蛋白芯片技术检测患者血清中幽门螺杆菌(Hp)毒素相关蛋白(CagA)、尿素酶(UreC)及热休克蛋白(Hsp60)抗体,并与胃黏膜标本快速尿素酶试验(RUT)和改良的Giemsa染色技术进行客观分析评价。方法对42例因上消化道不适就诊的儿童患者留取血清,深低温冻存同时作蛋白芯片检测和胃黏膜标本快速尿素酶试验(RUT)和改良的Giemsa染色检测。结果经χ2检验,蛋白芯片检测CagA,UreC,Hsp60抗体与胃黏膜活检组织快速尿素酶试验和Giemsa染色联合检测的结果对比P>0.05;儿童CagA+型菌感染仅占21.4%。结论蛋白芯片技术与胃黏膜活检标本联合检测相比,检测结果差异无显著性;蛋白芯片技术能对Hp菌株感染分型及现症感染鉴定,具有快速,准确,检测指标多等优点,是一种很有发展前途的检测技术,值得推广应用。
OBJECTIVE To evaluate the diagnostic technology of Helicobacter pylori （Hp） infection in children. METHODS Serum samples were taken and frozen at -70℃ from 42 patients for detection Hp infection with protein chips technology. Antral biopsy specimens obtained by gastroscopy were detected with modified Giemsa stain and rapid urease test （RUT）. RESULTS （1）These detecting methods were evaluated by x^2 test. The result of modified Giemsa stain detection combined with RUT was similar to that of serum IgG antibody examination of cytotoxin associated gene protein A CagA, urease and heat shock protein Hsp60 of H. pylori infection by protein chips technology （P〉0.05）. （2）CagA-positive H. pylori strains of children were only 21.4%. CONCLUSIONS （1）The detection of protein chips technology against H. pylori infection is compared with antral biopsy specimens. The result is without significant difference. （2） Protein chips technology can discover different types of H. pylori strains infection and determine present infection. It is a rapid, accurate method and can detect more factors. It＇s a potential detecting method and worthy of using widely.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2006年第4期466-468,共3页
Chinese Journal of Nosocomiology
基金
湖北省卫生厅基金资助项目(301140296)
关键词
幽门螺杆菌
儿童
Helicobacter pylori
Children
