摘要
目的:探讨尿激酶型纤溶酶原激活剂(uPA)基因启动子甲基化状况与喉癌侵袭转移的关系。方法:采用RT-PCR法检测40例喉癌组织中uPAmRNA表达;同时用甲基化特异性PCR(MSP)法检测uPA基因启动子甲基化状况。结果:uPA基因表达率为65.0%(26/40);有颈淋巴结转移的喉癌与无颈淋巴结转移的喉癌比较,uPA基因表达率显著增高(P<0.01)。uPA基因启动子甲基化率为20.0%(8/40);有颈淋巴结转移的喉癌与无颈淋巴结转移的喉癌比较,uPA基因启动子甲基化率显著降低(P<0.01)。甲基化的喉癌组织中均无uPA基因表达。结论:uPA基因启动子甲基化是uPA基因表达缺失的机制之一,uPA基因启动子的去甲基化机制可能与喉癌颈淋巴结转移有关。
Objective：To investigate the relationship between methylation status of urokinase type plasminogen activator （uPA） promoter and invasion/metastasis of laryngeal squamous cell carcinoma （LSCC）. Method： Using RT--PCR technique, specimens from 40 patients of LSCC were detected for their expression of the uPA gene. Meanwhile, methylation-specific PCR was used to detect methylation status of uPA promoter. Result：Positive expression rate of uPAmRNA in LSCC was 65. 0%（26/40）, and the rate of expression of uPAmRNA in LSCC with cervical lymph node metastasis was higher than that of in LSCC without cervical lymph node metastasis （ P〈0.05）. Methylation of uPA promoter was observed in 8 （20.0%） of 40 cases of LSCC, and the rate of methylation of uPA promoter in LSCC with cervical lymph node metastasis was lower than that of in LSCC without cervical lymph node metastasis（ P〈0.01）. The expression of uPAmRNA was not detected in methylated cancer tissues. Conclusion：Methylation leads to lose of the uPA gene expression in LSCC. Demethylation of uPA promoter may be correlated to cervical lymph node metastasis.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第9期409-411,共3页
Journal of Clinical Otorhinolaryngology
