摘要
目的建立测定奥美拉唑血药浓度的反相高效液相色谱法，考察样品提取及色谱分离条件。方法血浆中加入内标尼美舒利后，用以乙醚提取，进行高效液相色谱法检测。以Dikma Technologies C18柱，甲醇：水：三乙胺：冰醋酸（60：40：0．5：0．5）为流动相进行色谱分离。流速1．0ml／min，检测波长302nm。结果奥美拉唑浓度在50—1800ng/ml范围内线性良好，r=0．9995。测定浓度为15、100、1500ng/ml的血浆样品，其日间及日内的RSD分别为0．86％、6．7％、4．93％和3．04％、4．25％、5．22％（n=5）。3种浓度的提取回收率分别为77．74％、79．44％和86．30％。结论方法简便、准确、灵敏，可用于奥美拉唑血药浓度的测定。
Objective A reversed-phase high performance liquid chromatography （RP-HPLC） was used to dertermine the concentration of omeprazole in human plasma. Meanwhile the sample extraction and chromatography isocation condition were examined. Methods After adding the internal standard nimesulide, the plasma samples were extracted with ethyl ether and determined by HPLC. Waters instrument was used with the column of Dikma Diamonsil C18 （200mm × 4.6mm,5μm）. The mobile phase was compound of methanol-water-triethylamine-glacial acetic acid（60：40：0.5：0.5,V/V）. How rate was 1.0ml/min. Results The omeprazole was quantitated using a ultraviolet detector operated at 302nm which allowed determinater at 5 - 1 800ng/ml of omeprazole with high reproduciblicity （r = 0. 9995）. The with-inday and between-day precision（RSD） of the concentration（15,100,1 500ng/ml） were 0.86% ,6.7% ,4.93% and 3.04% ,4.25% ,5.22%（n = 5）%, respectively. The recoveries of omeprazole were 92.21% - 105.3% for plasma. Conclusion The RP-HPLC method is simple, rapid and sensitive, It is applicable to determine the concentration of omeprazole in huaman plasma.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2006年第2期170-172,共3页
Journal of Harbin Medical University
