摘要
目的 为寻找更多有效的旋毛虫病疫苗候选抗原分子，构建旋毛虫成虫部分cDNA质粒文库。方法 以旋毛虫成虫cDNA文库为模板，进行PCR扩增。扩增产物500-2000bp之间的DNA片段用BamHⅠ、HindⅢ酶切，将酶切产物克隆入真核表达载体pcDNA3．1，对部分转化子进行酶切鉴定。结果 构建了插入片段在500-1500 bp之间的旋毛虫成虫部分cDNA质粒文库，90％以上的转化子都含有插入的DNA片段。结论 成功构建一个旋毛虫成虫部分cDNA质粒文库。
Objective To find out more effective vaccine candidate antigen against the Trichinella spiralis, and to construct the adult cDNA plasmid library of T. spiralis. Methods The cDNA fragments were amplified using adult cDNA plasmid library as the template by PCR and then the fragments digested with BamH Ⅰ and Hind Ⅲ to the size of 500-1 500 bp were cloned into a eukaryotic expression vector pcDNA3. 1. The inserted DNA fragments in the recombinants were identified by digestion with restriction enzyme. Results The partial adult cDNA plasmid library of T. spiralis, with -10^5 transformants, was constructed. Most of the recombinants contained the insert DNA fragments. Conclusion A partial adult cDNA plasmid library of T. spiralis is constructed successfully.
出处
《首都医科大学学报》
CAS
2006年第2期155-157,共3页
Journal of Capital Medical University
基金
美国中华医学基金(CMBParasitology98-674)
北京市教委科技发展计划(KM200410025001)资助项目
关键词
旋毛虫
cDNA表达质粒文库
构建
Trichinella spiralis
cDNA expression plasmid library
construction
