摘要
目的:研究建立较为理想的寒湿痹型实验性类风湿关节炎模型。方法:采用不同浓度Ⅱ型胶原和弗氏佐剂联合应用于大鼠足部注射,诱导关节炎;测量大鼠足肿胀度,并计算肿胀率;检测血清唾液酸水平;观察滑膜组织病理学及超微结构变化。结果:低浓度Ⅱ型胶原和完全佐剂组大鼠关节肿势迅猛,近似于佐剂关节炎大鼠模型,高浓度Ⅱ型胶原和不完全佐剂组大鼠关节肿势和缓;各模型组大鼠血清唾液酸水平均高于正常对照组;滑膜组织的病理学变化与类风湿关节炎相符。结论:高浓度的Ⅱ型胶原加不完全佐剂给药组是较为理想类风湿关节炎大鼠模型,中医辩证属寒湿痹症,与之相比较,佐剂关节炎模型接近于湿热痹症。
Objective：Research on establishing a better experimental arthritis rat model of cold-dampness arthraligia on rheumatoid arthritis（RA） research. Methods：In order to induce arthritis, the rat＇s feet were injected with different concentrations of type Ⅱ collagen mixed with Freund＇s adjuvant;measure the feet swelling degrees of rats, then calculated swelling rate;detect the level of serum sialic acid （SA） ;observ the synovial histopathology and ultrastructural of rats. Results：The rat joints swell of the group with low concentration of type Ⅱ collagen and completed Freund＇s adjuvant was rapid and severe ,while that of the group with high concentration of type Ⅱ collagen and incompleted Freund＇s adjuvant was moderate and lasting. The level of serum SA of model groups was higher than the normal group. The histopathology changes of rat＇s synovium conform to RA. Conclusion：The group injected with high concentration of type Ⅱ collagen and incompleted Freundg adjuvant is the ideal RA model. It is belonged to the symptom of cold-dampness arthralgia, compared with this model, the AA approaches the symptom of heat-dampness arthralgia.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第4期350-352,共3页
Chinese Journal of Immunology
