摘要
目的240个汉族无关个体12个STR位点基因频率调查及其法医学应用;方法采用12位点复合扩增及变性聚丙烯酰胺凝胶电泳基因分型;结果该系统12个STR基因位点在汉族人群中均为高识别率位点,特别适合于陈旧血痕检验;结论12位点STR-PCR复合扩增系统检测方法简便,经济实用,在法医个体识别和亲子鉴定中具有应用价值。
Objective To investigate the genetic polymorphism of 12 STR loci in 240 Han population and evaluate application of the 12 STR loci systems in forensic cases. Methods 12 STR loci were amplified with Muti-loci STR PCR system, which were genotyped with electrophoresis separation on dPAGE. Results 12 STR loci system had high power of discrimination (DP) and it was suited for analysis of dated bloodstain especially. Conclusion The 12 STR loci systems is simple economy and practicality, which is useful in forensic identification & disputed paternity.
出处
《法医学杂志》
CAS
CSCD
2006年第2期120-121,共2页
Journal of Forensic Medicine
关键词
STR—PCR
等位基因频率
银染
short tandem repeat(STR)
allelic frequency
sliver stain