摘要
研究了DBM-偶氮胂（DBM—ASA）与血清白蛋白（BSA）的反应。在室温下，于pH2．9的Britton—Robinson缓冲溶液中及乳化剂OP存在下，DBM—ASA与血清白蛋白复合物最大吸收峰位于530nm处，复合物表观摩尔吸光系数εmax=5．73×10^3L／（mol·cm），BSA在浓度10．0～75．0mg／L范围内呈良好线性关系。加入乳化剂OP可显著提高体系灵敏度与稳定性。加入少量乙醇能提高反应灵敏度。用本法测定了人血清样品中的蛋白质含量，结果满意。
The reaction between DBM-arsenazo and bovine serum albumin was studied. In a Britton-Robinson buffer solution at pH 2.9 and in the presence of emulgent OP, DBM-arsenazo and bovine serum albumin form a compound with a maximum absorption wavelength at 530 nm. The apparent molar absorptivity of the compound is εmax = 5.73 × 10^5 L/ （mol＇em）. The linear range of bovine serum albumin is 10 ×75.0 mg/L. The addition of emulgent OP can obviousl） enhar.ee the sensitivity and stability of the eolour system. The addition of a small amount of alcohol can increase the sensitivity of the eolour system. The protein in human serum was determined by the proposed method with satisfactory results.
出处
《化学世界》
CAS
CSCD
北大核心
2006年第4期217-219,共3页
Chemical World
