摘要
目的通过COX-2抑制剂NS-398对胃癌培养细胞系SGC7901增殖及凋亡影响的研究证实非甾体类抗炎药(NSAIDs)可在体外抑制胃癌细胞的生长。方法应用MTT法检测NS-398对胃癌细胞SGC7901细胞增殖的影响;应用流式细胞仪、透射电镜检测NS-398对胃癌细胞SGC7901细胞凋亡的影响。结果MTT结果显示NS-398对胃癌细胞均抑制作用,并随剂量的增加及作用时间延长,其抑制作用要明显增加;透射电镜观察发现未经NS-398作用的胃癌培养细胞的细胞核和细胞器亚微结构清晰,核模完整,而经不同浓度的舒林酸和尼美舒利作用后细胞呈现典型的凋亡形态学变化,并可见凋亡小体形成;流式细胞仪结果显示不同浓度的NS-398作用SGC7901细胞72 h后,均可出现亚G1峰,呈剂量依赖关系诱导胃癌细胞凋亡,并呈浓度依赖性改变的细胞周期分布,一方面增高G0/G期细胞比例,另一方面降低S期和G2/M期细胞比例。结论NS-398可抑制胃癌SGC7901细胞的增殖;NS-398促进胃癌SGC7901细胞的凋亡;NS-398抑制胃癌的机制可能是通过抑制胃癌细胞COX-2的活性,从而抑制前列腺素E2的释放而抑制胃癌细胞的生长。
Objective To confirm NS-398 can inhibit growth of gastic cancer cell line in vitro, and to investigate the mechanism of NSAIDs＇ effect on anti-gastric cancer by studying the influence of NS-398 on inducing growth inhibition and apoptosis in human gastric cancer. Methods Proliferation and apoptosis were measured by MTT assay and electron microscope and Flow cytometric. Results MTT assay showed NS-398 could inhibit the growth of gastric cancer in dose and time dependent manner. Flow cytometric analysis and electron microscope showed that NS-398 could induce apoptosis of gastric cancer cell line SGC7901 in dose and time dependent manner. Conclusion NS-398 can inhibit the cell proliferation and induce cell apoptosis in gastric cancer. The mechanism of NS-398 on inhabiting the growth of gastric cancer was that by inhibiting the activity of COX-2 and decrease the PGE2 releasing to inhibit the cell proliferation.
出处
《中国实验诊断学》
2006年第4期369-371,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅科研资助项目(20010707)
