摘要
在间歇发酵基础上,在工作体积为2 L的生物反应器中,对一株植物乳酸杆菌RS2 2进行了连续发酵研究.结果表明,当稀释率为0.06 h-1时,平均酶活产率达到1 305 U/L,菌体浓度达到1.12 g/L,体积产率为137.4 U.L-1.h-1,是间歇发酵(52.1 U.L-1.h-1)的2.63倍,发酵时间为100 h.还原糖利用率为86%.
The continuous fermentation Lactobacillus plantarum RS2 - 2 in 2 L bioreactor was studied on the basis of batch fermentation. The results showed that the activity of LDH could reach 1305 U/L, the biomass could reached 1.12 g/L , overall productivity could reach 137.4 U·L^-1· h^-1 with this continuous fermentation system over 100 h at a dilution rate of 0.06 h^-1. The produce ratio was 2.63 times of that with batch fermentation （52.1 U·L^-1. h 41）. The consumption ratio of substrate was 86 % in the continuous fermentation.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2006年第2期43-46,共4页
Journal of Henan University of Technology：Natural Science Edition
关键词
植物乳酸杆菌
乳酸脱氢酶
连续发酵
Lactobacillus plantarum
lactate dehydrogenase
continuous fermentation
