摘要
目的探讨促红细胞生成素(EPO)在脑血管痉挛病理过程中的作用。方法 27只健康成年狗随机分为阴性对照组、单纯注血组和EPO治疗组,于动物模型制作成功后1 h(0 d)、3 d、7 d 和14 d采集脑脊液及血浆标本,测定其内皮素(ET—1)和一氧化氮(NO)含量变化。结果单纯注血组第3天开始ET—1含量明显高于阴性对照组(P<0．05),持续到第7天后逐渐下降,在第14天基本恢复正常;EPO治疗组在3 d、7 d血浆和脑脊液中ET-1含量稍高于对照组(P>0．05),但与单纯注血组比较显著性降低(P<0．05)。单纯注血组血浆和脑脊液NO浓度显著性下降(P<0．01)。EPO治疗组各时间点NO 检测与阴性对照组相比,差异没有显著性(P<0．05)。处理因素与时间之间存在交互作用。结论 EPO 能够显著改善脑血管痉挛发生时ET-1和NO的变化,在脑血管痉挛发生中具有重要的保护作用。
Objective To explore the role of erythropoietin in the development of brain vascular spasm in dog. Methods Twenty-seven healthy adult dogs were randomly divided into 3 groups, negative control group, blood injective group and EPO therapeutic group. Endothelin（ET-1） and Nitric oxide（NO） of blood plasma and cerebrospinal fluid were measured at time of 1h （0d）,3 d ,7 d and 14 d after the dog vascular spasm models were made. Results The concentration of ET-1 began to increase more obviously in blood injective group on the third day than that in negative group （P〈0.05）. After 7 days, the concentration of ET-1 began to decrease gradually and revert to be normal after 14 days. The concentration of ET-1 in EPO therapeutic group in blood plasma on the third day and the seventh day and CSF was a bit higher than that in control group（P〉0.05）, but has less obvious difference from that in blood injective group （P〈0.05）. There is no significant difference between NO test of EPO therapeutic in different time and negative control group （P〉0.05）. The treatment factors and time duration take effect interactively. Conclusion EPO may amend the change of ET-1 and NO in the development of brain vascular spasm, which demonstrates EPO takes an important protective role to be against brain vascular spasm.
出处
《中华神经医学杂志》
CAS
CSCD
2006年第4期366-368,共3页
Chinese Journal of Neuromedicine
